Problem

Solving the sequence of an oligopeptide from sequence analysis dataAmino acid analysis of...

Solving the sequence of an oligopeptide from sequence analysis data

Amino acid analysis of an octapeptide revealed the following composition:

2 Arg

1 Gly

1 Met

1 Trp

1 Tyr

1 Phe

1 Lys

The following facts were observed:

a. Edman degradation gave
b. CNBr treatment yielded a pentapeptide and a tripeptide containing phenylalanine.
c. Chymotrypsin treatment yielded a tetrapeptide containing a C-terminal indole amino acid and two dipeptides.
d. Trypsin treatment yielded a tetrapeptide, a dipeptide, and free Lys and Phe.
e. Clostripain yielded a pentapeptide, a dipeptide, and free Phe.