Using graphical analysis to determine the KD for the interaction between a protein and its ligand
A quantitative study of the interaction of a protein with its ligand yielded the following results:
Ligand concentration (mM) | 1, | 2, | 3, | 4, | 5, | 6, | 9, | 12 |
v (moles of ligand bound per mole of protein) | 0.28, | 0.45, | 0.56, | 0.60, | 0.71, | 0.75, | 0.79, | 0.83 |
Plot a graph of [L] versus v. Determine KD, the dissociation constant for the interaction between the protein and its ligand, from the graph.
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