Effects of temperature on ethanol production. A lowcost and highly productive bio-fuel production method is high-temperature fermentation. However, heat stress can inhibit the amount of ethanol produced during the process. In Engineering Life Sciences (Mar. 2013), biochemical engineers carried out a series of experiments to assess the effect of temperature on ethanol production during fermentation. The maximum inhibitory concentration of ethanol (grams per liter) was measured at four different temperatures (30°, 35°, 40°, and 45° Celsius). The experiment was replicated 3 times, with the data shown in the table. (Note: The data are simulated based on information provided in the journal article.) Do the data indicate that high temperatures inhibit mean concentration of ethanol? Test using α= .10.
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