The kinetics of double-strand formation for a DNA oligo nucleotide containing a G-T base p...

The kinetics of double-strand formation for a DNA oligo nucleotide containing a G-T base pair was measured by temperature-jump kinetics. The reaction is

The following data were obtained:

a. Determine Ea, ∆H‡ and ∆S‡ for the forward and reverse reactions; assume that the values are independent of temperature.

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b. Determine the standard enthalpy ∆H° and standard entropy ∆5° changes for the reaction (assumed inde pendent of temperature) and calculate the equilibrium constant for duplex formation at 37 °C.

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c. Discuss qualitatively what you would expect to happen to ∆H‡ and the standard enthalpy change for the reaction if the duplex contained one more Watson-Crick base pair. Would each increase, decrease, or remain the same?