Homework Answers
Solution:
>>>The activity of an enzyme increases with increase in temperature initially but after some time enzyme will becomes denatures and enzyme activity diminished. Thus, the at 100 °C, the activity of enzyme diminished due to denaturation.
>>> The decrease in temperature upto 5 °C, causes freezing of enzyme and enzyme activity decreased but not diminished because when temperature of the enzyme becomes normal, it gains its activity.
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3. You did some research after the Enzyme lab and you found out that the optimal...
You recently joined a biochemistry research lab to study protein functions in diseases. In the lab,...
You recently joined a biochemistry research lab to study protein functions in diseases. In the lab, researchers have been working on a protein kinase, the enzyme that catalyzes the phosphoryl transfer reaction from ATP to the -OH group on serine or threonine residues of the target proteins. The researchers in the lab have found that a point mutation in the gene encoding the protein kinase leads to a change in one amino acid of the enzyme, which causes an increase...
A. Your lab To see if you understand what you did in our lab, answer the...
A. Your lab To see if you understand what you did in our lab, answer the following based in the procedures for the restriction digest and the gel electrophoresis. 1. Using the PGLO map on p7 of the gel electrophoresis procedure, predict the size of the fragments generated by each enzyme EcoRI Hindill, and Pst! (the sizes you would expect to see on the gel.) (6 pts) Hindill -8 fragments were produced by the restriction enzyme. 2. Answer the calculation...
Help on prelab NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
4.) The enzyme, pepsin, is one that hudrolvess proteins in the stomach, where the pH Is...
4.) The enzyme, pepsin, is one that hudrolvess proteins in the stomach, where the pH Is aboun 2 due to stomach acidx, into individual amino acids a) What would you expect to happen so the activity of the enzyme pepsin when in exits the stomach and enters the small imestine where the pi is about ? b.) Amylase is found in saliva where hydrolysis of starches begins. What would you expeet to happen to the activity of the enryme amylase...
3. You are an undergraduate research in my lab and are asked to perform an experiment...
3. You are an undergraduate research in my lab and are asked to perform an experiment is in which you infect a mouse with a virus to induce a CTL response. a. Before you start your experiment, demonstrate to me that understand what is happening between the naïve T cell that will be activated during the viral response and the dendritic cell that activates it. Draw the immunological synapse between these two cells with all of the proteins involved. Label...
how does changing saline concentration affect enzyme activity? (only saline not ph) what is the hypothesis...
how
does changing saline concentration affect enzyme activity? (only
saline not ph)
what is the hypothesis
Enzyme Activity Design Investigation The compound hydrogen peroxide, Hou by product of the reaction in most living organisms. However, Hydrogen peroxide is damaging to delicate molecules inside ces As a result, nearly all organisms produce the enzyme catalase, which breaks down H,O, it is formed Potatoes are one source of catalase Catalase speeds up the breakdown of hydrogen peroxide into water and gaseoul sxygen...
Does anyone know how to do this? Thanks in advance! Enzyme Activity (ml) Effect of pH...
Does anyone know how to do this? Thanks in advance!
Enzyme Activity (ml) Effect of pH on (amylase) enzyme activity. For determination of optimum assay pH of the enzyme reaction, 0.05 M Na2HPO4-NaH2PO4 buffer was used. The reaction was carried out for 10 min at 50°C in a shaking water. The enzyme activity was measured and the results are presented on graph, Bars represent means standard deviations for three replicates. 1. What do the error bars in this graph represent?...
Post-Lab Questions For each of the tubing pieces, identify whether the solution inside was hypotonic, hypertonic,...
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Atropine blocks muscarinic receptors. Thus, it is a muscarinic antagonist. a. In what region of the...
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3) In lab 11, you found the relationship between the force exerted by a spring and...
3) In lab 11, you found the relationship between the force exerted by a spring and its length to determine the spring constant. You could also do this using simple harmonic motion. a. Describe an experiment you could do to find a spring constant using simple harmonic motion. What would you measure, and how would you average your measurements? b. Pretending you did the lab this way, make up reasonable measurements and compute the spring constant for those measurements.
A. Your lab To see if you understand what you did in our lab, answer the following based in the procedures for the restriction digest and the gel electrophoresis. 1. Using the PGLO map on p7 of the gel electrophoresis procedure, predict the size of the fragments generated by each enzyme EcoRI Hindill, and Pst! (the sizes you would expect to see on the gel.) (6 pts) Hindill -8 fragments were produced by the restriction enzyme. 2. Answer the calculation...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
4.) The enzyme, pepsin, is one that hudrolvess proteins in the stomach, where the pH Is aboun 2 due to stomach acidx, into individual amino acids a) What would you expect to happen so the activity of the enzyme pepsin when in exits the stomach and enters the small imestine where the pi is about ? b.) Amylase is found in saliva where hydrolysis of starches begins. What would you expeet to happen to the activity of the enryme amylase...
3. You are an undergraduate research in my lab and are asked to perform an experiment is in which you infect a mouse with a virus to induce a CTL response. a. Before you start your experiment, demonstrate to me that understand what is happening between the naïve T cell that will be activated during the viral response and the dendritic cell that activates it. Draw the immunological synapse between these two cells with all of the proteins involved. Label...
how
does changing saline concentration affect enzyme activity? (only
saline not ph)
what is the hypothesis
Enzyme Activity Design Investigation The compound hydrogen peroxide, Hou by product of the reaction in most living organisms. However, Hydrogen peroxide is damaging to delicate molecules inside ces As a result, nearly all organisms produce the enzyme catalase, which breaks down H,O, it is formed Potatoes are one source of catalase Catalase speeds up the breakdown of hydrogen peroxide into water and gaseoul sxygen...
Does anyone know how to do this? Thanks in advance!
Enzyme Activity (ml) Effect of pH on (amylase) enzyme activity. For determination of optimum assay pH of the enzyme reaction, 0.05 M Na2HPO4-NaH2PO4 buffer was used. The reaction was carried out for 10 min at 50°C in a shaking water. The enzyme activity was measured and the results are presented on graph, Bars represent means standard deviations for three replicates. 1. What do the error bars in this graph represent?...
Atropine blocks muscarinic receptors. Thus, it is a muscarinic antagonist. a. In what region of the conduction system would atropine bind to muscarinic receptors? b. In doing so, how would this affect the ability of pilocarpine to bind? c. What would you expect to happen to heart rate? d. Is that what you found in your experiment? If your experiment did not yield the results you expected, why do you think this may have happened?
3) In lab 11, you found the relationship between the force exerted by a spring and its length to determine the spring constant. You could also do this using simple harmonic motion. a. Describe an experiment you could do to find a spring constant using simple harmonic motion. What would you measure, and how would you average your measurements? b. Pretending you did the lab this way, make up reasonable measurements and compute the spring constant for those measurements.
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