Design an experiment around a mAb to ensure that a set number of patients died from...
Homework Answers
An experiment around a mAb to ensure that a set number of patients died from a Dengue infection would be to use the mAb against the Dengue envelope (E) to do an assay to confirm the serotype specificity of dengue viruses in conventional diagnosis as viral isolation. A positive control use of mAb with a known patient of Dengue. A negative control is the use of mAb with a normal healthy individual.
Add Answer to:
Design an experiment around a mAb to ensure that a set number of
patients died from...
III. Mapping the Field Around a Bar Magnet: An Observation Experiment Purposes: Design an experiment to take approp...
III. Mapping the Field Around a Bar Magnet: An Observation Experiment Purposes: Design an experiment to take appropriate data to find a relationship. Construct a mathematical model to describe that relationship Description: Your task is to use a compass to map the magnetic field of a small magnet. Construct a circle of radius 10 cm on a clean sheet of paper, and mark points on the circle every 30°. Place the bar magnet so that its center is resting at...
4. The foundation of experimental design comes from agriculture. Here is a typical experiment You want...
4. The foundation of experimental design comes from agriculture. Here is a typical experiment You want to study the effect of two fertilizers, sulfate of ammonia and superphosphate, on the yield of Brussels sprouts. Each fertilizer is used at four levels: 0, 30, 60, and 90 pounds per acre. (a) Describe the response variable (b) Describe the treatments. How many levels for each treatment? (c) List a complete set of treatment combinations. Arrange your list in table form (d) Suppose...
Design Layout References Maiings Review View Design Layout Telil me what Lab 6: Molecular Models Experiment:...
Design Layout References Maiings Review View Design Layout Telil me what Lab 6: Molecular Models Experiment: Molecular Structures Materials 3 Bar magnets 20 Toothpicks Modeling clay Protractor Procedure **Take photographs of your experimental set up for Part 1 and 2, and your results. Submit them with your laboratory report Part 1: Magnets 1. Bring together the same poles of two bar magnets Observe and describe what happens in Table 3. Explore the linear shape of molecules by bringing the same...
You hypothesize that your protein of interest moves from the mitochondria to lysosomes when a cell...
You hypothesize that your protein of interest moves from the mitochondria to lysosomes when a cell is exposed to the drug Fantasalin. A. Design a microscopy experiment to test this hypothesis. Please include the LABELS and type(s) of microscopy you would use. Also, please draw illustrations of what you predict your experiment to look like. B. Design an experiment to test this hypothesis using cell fractionation and SDS-PAGE. Please include what samples you would collect to run on the gel,...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was...
Suppose you have been asked by a food company to design an experiment that examines consumers’...
Suppose you have been asked by a food company to design an experiment that examines consumers’ purchase intentions toward their product (i.e., cereals), as a result of their recent pricing strategies (i.e., low price, high price) and product quality (i.e., organic offerings, nonorganic offerings) strategies. Basically, they would like to know the best combination of product quality offering and pricing strategy that yields the highest levels of consumers’ purchase intentions. How would this experiment look like? When working on this...
Please help 15. During our paper lab experiment, we compared the tumor cells from one patient...
Please help
15. During our paper lab experiment, we compared the tumor cells from one patient to the normal cells of the same patient. If we wanted to do a larger study to give a larger picture of what is going on in colon cancer cells which of the following would be a better way to set up the experiment? A. Collect cancer cells and normal cells from 1000 patients and run 1000 different microarrays (one for each person) and...
Biotechniques You are interested in studying the effects of glucose on Gene X. Your hypothesis is...
Biotechniques
You are interested in studying the effects of glucose on Gene X. Your hypothesis is that expression of Gene X will increase/decrease under high glucose conditions. To prove this, design 5 experiments using the following techniques: Reporter assay sensitivity assay EMSA blot In situ hybridization (IHC with probe for RNA) For each experiment, answer the following questions: What does the technique tell you (what does it measure)? How does this information help support your hypothesis (what part of the...
Keeping in mind the assays done in Lab 1/2, (a) how could you design a colorimetric...
Keeping in mind the assays done in Lab 1/2, (a) how could you design a colorimetric method to measure alpha-amylase activity? (b) What would you use as a control? (c) At what wavelength should the spectrophotometer be set? (d) Sketch a graph like Plot 1 (from this lab) to represent the amylase activity you would observe using your method. You want to know whether alpha-amylase is more active in the stomach (pH 2) or the intestines (pH 7). (a) How...
4. (Challenge) You are purifying a protein from the brain of patients with an unusual type of...
4. (Challenge) You are purifying a protein from the brain of patients with an unusual type of dementia triggered by a coronavirus and you hypothesize that this protein may be a signature molecule of this type of dementia. To pursue this hypothesis, you collect brain tissue from patients post mortem, do differential centrifugation, density gradient centrifugation, CM (carboxymethyl cellulose) ion exchange chromatography and gel filtration. You then assess the purity of your protein using both native and SDS gel electrophoresis. The...
III. Mapping the Field Around a Bar Magnet: An Observation Experiment Purposes: Design an experiment to take appropriate data to find a relationship. Construct a mathematical model to describe that relationship Description: Your task is to use a compass to map the magnetic field of a small magnet. Construct a circle of radius 10 cm on a clean sheet of paper, and mark points on the circle every 30°. Place the bar magnet so that its center is resting at...
4. The foundation of experimental design comes from agriculture. Here is a typical experiment You want to study the effect of two fertilizers, sulfate of ammonia and superphosphate, on the yield of Brussels sprouts. Each fertilizer is used at four levels: 0, 30, 60, and 90 pounds per acre. (a) Describe the response variable (b) Describe the treatments. How many levels for each treatment? (c) List a complete set of treatment combinations. Arrange your list in table form (d) Suppose...
Design Layout References Maiings Review View Design Layout Telil me what Lab 6: Molecular Models Experiment: Molecular Structures Materials 3 Bar magnets 20 Toothpicks Modeling clay Protractor Procedure **Take photographs of your experimental set up for Part 1 and 2, and your results. Submit them with your laboratory report Part 1: Magnets 1. Bring together the same poles of two bar magnets Observe and describe what happens in Table 3. Explore the linear shape of molecules by bringing the same...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was...
Please help
15. During our paper lab experiment, we compared the tumor cells from one patient to the normal cells of the same patient. If we wanted to do a larger study to give a larger picture of what is going on in colon cancer cells which of the following would be a better way to set up the experiment? A. Collect cancer cells and normal cells from 1000 patients and run 1000 different microarrays (one for each person) and...
Biotechniques
You are interested in studying the effects of glucose on Gene X. Your hypothesis is that expression of Gene X will increase/decrease under high glucose conditions. To prove this, design 5 experiments using the following techniques: Reporter assay sensitivity assay EMSA blot In situ hybridization (IHC with probe for RNA) For each experiment, answer the following questions: What does the technique tell you (what does it measure)? How does this information help support your hypothesis (what part of the...
Keeping in mind the assays done in Lab 1/2, (a) how could you design a colorimetric method to measure alpha-amylase activity? (b) What would you use as a control? (c) At what wavelength should the spectrophotometer be set? (d) Sketch a graph like Plot 1 (from this lab) to represent the amylase activity you would observe using your method. You want to know whether alpha-amylase is more active in the stomach (pH 2) or the intestines (pH 7). (a) How...
Most questions answered within 3 hours.
-
Given the following information:
acetic acid
CH3COOH
Ka = 1.8×10-5
triethylamine
(C2H5)3N
Kb = 5.2×10-4
(1)...
asked 7 minutes ago -
Potassium permanganate(KMNO4)is has a solubility of 6.4 g/ 100 g
of water at 20ºC, and 250...
asked 4 minutes ago -
51.
As the marginal propensity to expend rises, the multiplier:
decreases.
is impossible to determine.
increases....
asked 10 minutes ago -
The Baldwin Company currently has the following balances on their
balance sheet:
Total
Liabilities
$69,309
Common...
asked 13 minutes ago -
A quiet town in Kansas has 10 people, all of whom have the same
preferences. There...
asked 19 minutes ago -
Summarize what an organization needs from a leader.
a. Analyze what might happen to an organization...
asked 22 minutes ago -
How would one critically evaluate an organizations marketing
strategies from the viewpoint of its consumers, as...
asked 20 minutes ago -
Given a standardized normal distribution (with μ = 0 and a σ =
1), what is...
asked 21 minutes ago -
Company XYZ know that replacement times for the quartz time
pieces it produces are normally distributed...
asked 36 minutes ago -
A) Write the acid-base reaction that occurs between Na2HPO4 and
NaOH
B) Write the possible reactions...
asked 49 minutes ago -
What advantages does the Natural Law Theory have in comparison
with the Divine Command Theory? Explain...
asked 55 minutes ago -
A diver comes off a board with arms straight up and legs
straight down, giving her...
asked 54 minutes ago