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QUESTION 39 TREGS express what transcription factor gene? OBAC-1 O Bcl-1 OCD59 O...
1) You have transfected siRNA against transcription factor A into cells that normally express the transcription...
1) You have transfected siRNA against transcription factor A into cells that normally express the transcription factor. These cells also contain a luciferase reporter gene that is controlled an enhancer that has multiple binding sites for transcription factor A. a. What would be the effect on reporter gene activity b. How could you determine whether RNAi acts at the level of mRNA or translation in this case?
2. a) Sketch a eukaryotic gene (brns) that is regulated by one transcription factor - Brt...
2. a) Sketch a eukaryotic gene (brns) that is regulated by one transcription factor - Brt - that bind 50 bps upstream of the transcription start site and an enhancer - Ned - that binds 10 kb upstream of the transcription start site. In your sketch, indicate the start of transcription, TATA box beginning of the open reading frame for your gene (ATG), location of the promoter, location of the transcription factors, location of the RNA polymerase II, location of...
The hunchback gene has an enhancer containing 3 binding sites for the transcription factor Bicoid. How...
The hunchback gene has an enhancer containing 3 binding sites for the transcription factor Bicoid. How could the loss of a single DNA binding site -leaving the 2 others intact- act to reduce gene expression of hunchback by more than 1/3 ? A. Bound Bicoid transcription factor molecules interact antagonistically to cause maximal gene expression B. Bound Bicoid transcription factor molecules interact additively to cause maximal gene expression C. Bicoid does not bind to the hunchback enhancer D. Bound Bicoid...
Question 20 TFIID is a. a protein and a transcription factor. b. an enzyme. c. a...
Question 20 TFIID is a. a protein and a transcription factor. b. an enzyme. c. a promoter and a transcription factor. d. a promoter. e. part of the TATA box. Question 19 Without maintenance methyltransferase, the changes made by DNA methyltransferase would not a. be able to increase transcription of a gene. b. be passed on from one generation to the next c. coordinate regulation across different genes. d. produce microRNA. e. be able to decrease transcription of a gene....
1) The FOXP2 transcription factor is expressed in human brain, heart, and lungs. The protein is...
1) The FOXP2 transcription factor is expressed in human brain, heart, and lungs. The protein is required for normal development. Given that eukaryotic genes do not occur in operons, how can the STAT 1 transcription factor simultaneously regulate the transcription of more than 275 different target genes? 2) Many genes are transcribed constitutively, while others are regulated specifically. Regulation can occur in response to various stimuli in either a positive or negative direction. Describe one type of gene that would...
O 2. Click on the + signs in each box on the right. Your goal is...
O 2. Click on the + signs in each box on the right. Your goal is to make the cells as green as possible by maximizing production of GFP What change can you make to each of the following to maximize expression of the GFP gene? a. Positive Transcription Factor Concentration b. mRNA Destroyer (exonuclease) Concentration: c. Positive Transcription Factor Affinity for the Gene Regulatory Sequence d. RNA polymerase Affinity for the Gene Regulatory Sequence e. Protein Degradation
Carvas X CO Inactivation of a specific transcription factor Modifications to the RNA transcript OPresence of...
Carvas X CO Inactivation of a specific transcription factor Modifications to the RNA transcript OPresence of repressor protelns to reduce transcription Question 8 10pts The following lists many forms in which gene expression ls regulated in Eukaryotes. Which of those is common to both prokaryotes and eukaryotes? Tight colling of the DNA around histone proteins, making it inaccessible to RNA polymerase Protecting the mRNA by adding a 5 G-cap and 3 poly A tal O Repulating the availablity of transcription...
QUESTION 1 Hox genes are genes that code for transcription factors. What do transcription factors do?...
QUESTION 1 Hox genes are genes that code for transcription factors. What do transcription factors do? A. Bind to DNA to make it available for transcription B. Make up specific body parts C. Activate ribosomes QUESTION 2 Genes that need to be turned on at the same time (for example, the genes that encode the proteins that build toes) will all respond to the same transcription factor. A. True B. False QUESTION 3 Hox genes are sometimes called "master switches"....
Question 11 (1 point) An O( C) mutation results in O no transcription. inducible transcription. Otranscription...
Question 11 (1 point) An O( C) mutation results in O no transcription. inducible transcription. Otranscription but no translation. no translation. constitutive transcription.
What level of protein structure is correct for the general transcription factor TFIID? Consider the follow...
What level of protein structure is correct for the general transcription factor TFIID? Consider the follow segment of an mRNA, ggguuaugacgacccccaauaaaggaaacaag... What would be the amino acid sequence? From which human gene is the above mRNA transcribed?
Question 20 TFIID is a. a protein and a transcription factor. b. an enzyme. c. a promoter and a transcription factor. d. a promoter. e. part of the TATA box. Question 19 Without maintenance methyltransferase, the changes made by DNA methyltransferase would not a. be able to increase transcription of a gene. b. be passed on from one generation to the next c. coordinate regulation across different genes. d. produce microRNA. e. be able to decrease transcription of a gene....
O 2. Click on the + signs in each box on the right. Your goal is to make the cells as green as possible by maximizing production of GFP What change can you make to each of the following to maximize expression of the GFP gene? a. Positive Transcription Factor Concentration b. mRNA Destroyer (exonuclease) Concentration: c. Positive Transcription Factor Affinity for the Gene Regulatory Sequence d. RNA polymerase Affinity for the Gene Regulatory Sequence e. Protein Degradation
Carvas X CO Inactivation of a specific transcription factor Modifications to the RNA transcript OPresence of repressor protelns to reduce transcription Question 8 10pts The following lists many forms in which gene expression ls regulated in Eukaryotes. Which of those is common to both prokaryotes and eukaryotes? Tight colling of the DNA around histone proteins, making it inaccessible to RNA polymerase Protecting the mRNA by adding a 5 G-cap and 3 poly A tal O Repulating the availablity of transcription...
Question 11 (1 point) An O( C) mutation results in O no transcription. inducible transcription. Otranscription but no translation. no translation. constitutive transcription.
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