Question

To observe the localization of a proteins in living bacterial cells, you can use:

1. Centrifugation
2. NMR
3. Gelelectrophoresis
4. GFPtranscriptionalfusions

5. GFPtranslationalfusions

6. 4&5

Answer 1

centrifugation separates the components in the cell by density gradient, NMR is used to identify the molecular structure, Gel electrophoresis helps to separate charged molecules on the gel by applying the electric field.

translational fusions are constructs having a gene of interest tagged with a reporter gene, if the reporter gene is GFP, the protein product will be the normal protein fused with GFP, translational fusion helps to localize the proteins in the cell.

the Transcriptional fusion is the promoter of the gene fused with a reporter gene, using transctiptional fusion , we can identify which cells express the genes.

so the answer is 5) GFP translational fusions.