Answer the question (1) What is the reasoning why basic dyes stain the bacterial cells? (2)...
Answer the question
(1) What is the reasoning why basic dyes stain the bacterial cells?
(2) Immersion oil is used on the bright-field microscope to observe bacterial cells. What objective can only be used with the immersion oil?
Homework Answers
1) basic dyes are positively charged and the bacterial cell wall is negatively charged so basic dyes can stain the bacterial cell walls more efficiently because positive and negative charges attract each other.
2) resolution of is the minimum distance (r) between 2 points such that the 2 points can be distinguished as 2 separate points,
r= 1.22
/2nsin
,
where = wavelength of
light, 2nsin= numerical
aperture of the lens
n is the refractive index
oil has greater refractive index than air ( n of air=1) if we are using oil as the medium, refractive index of medium increases and r is inversely proportional to n so as n increases r decreases,
so resolving of the microscope increases so we can clearly see the the bacteria.
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Answer the question
(1) What is the reasoning why basic dyes stain the bacterial
cells?
(2)...
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