Question

2) On your first day working in my lab, you obtain the following DNA sequence: 3 AATTATACACGATGAAGCTTGTGACAGGTTTCCAATCATTAA

Codons Found in Messenger RNA Second Base Phe SerTyr Cys U Phe SeTy Cys C Leu Ser Stop Stop A Leu SerStop Trp G Leu Pro His A

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Answer #1

In a mRNA molecule, the start codon is always the AUG from the 5' site, i.e. 5' AUG 3'. This codon encodes for amino acid methionine in all the organisms. Further, the codon immediately after the start codon represents the second codon and the triplets are used for encoding the amino acid based upon the codon table. Finally, translation stops when a stop codon is encountered i.e. UAG, UGA or UAA.

These parameters are similar for all the mRNAs including the Drosophila as well as human p53.

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