The natural inducer of lac operon is [B] allolactose.
Allolactose binds to the repressor and decreases the repressor's affinity for the operator site.
The natural inducer for the lac operon is A) lactose B) allolactose C) ?-galactosidase D galactose...
The enzyme b-galactosidase converts the disaccharide lactose into? A. allolactose B. Glucose C. allolactose and glucose D. allolactose, glucose, galactose E. sucrose and glucose
The inducer of the lactose operon in E. coli is CAP. cAMP. allolactose. glucose. galactos Codons refer to triplet base sequences in the mRNA. nontemplate strand of DNA. template strand of DNA. tRNA. rRNA.
In the presence of lactose an inducer molecule binds to the lac repressor molecule. the lac repressor binds to the operator. genes of the lac operon are not expressed. all of the choices.
You are asked to develop a demonstration to show how the lac operon works. You decide to use X-gal and IPTG to determine if the enzyme ?-galactosidase is active. X-gal is a lactose analog that turns blue when metabolized by ?-galactosidase, but it does not induce the lac operon. IPTG is an inducer of the lac operon, but is not metabolized by ?-galactosidase. a. (2pts) Which of the following would you expect to bind to ?-galactosidase. Circle all that apply....
for 1-5 define those The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
The lac operon contains three genes: lacZ, lacY, and lacA for β‑galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively. The operon also contains a promoter site and an operator site. The regulatory gene includes the I gene for the Lac repressor protein and a promoter for that gene.The lac operon undergoes negative regulation. In the normal condition, the Lac repressor protein is active. Allolactose, an isomer of lactose, is the signal molecule that binds to the Lac repressor. Determine which events...
The diagram below illustrates the LAC operon in its OFF state when the inducer molecule —lactose—is absent. Predict the ways in which the following conditions will affect the transcription of the lactose-utilization genes. OPERON Regulatory Promoter Operator_ gene Lactose-utilization genes DNA mRNA RNA polymerase cannot attach to promoter Active repressor Protein If a mutation in the regulatory gene results in a misfolding of the repressor protein so that it can no longer bind DNA, the lactose-utilization genes O Will be...
To study the lac operon in E. coli, you added IPTG in the growth media, which is another analog of allolactose that is an inducer of the lac operon but is not metabolized by β-gal. You measured the activity of the enzyme β- galactosidase (β-gal) by X-gal. After mutagenesis (induce mutations) you find mutants that never turn blue, which DNA component(s) could produce such phenotype?
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site in DNA, thus allowing for expression of the genes coded by the Lac Operon. However, lactose cannot cross the bacterial cell membrane without the help of the Permease protein channel, which is coded by the lacY gene in the Lac Operon. So, it appears that we have a case of "what's first, the chicken or the egg?" - how can lactose enter the cell...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz