Homework Answers
DNA topoisomerases are known to play active role in unwinding replication fork of DNA. Each turn of helix of Eukaryotic DNA comprises of 10 base pairs, and during replication after its duplication at replication fork the chromosome ahead of the fork has to rotate.
Coming to calculations: 
100 base pairs are being synthesized per second, This means 6000 base pairs are being synthesized per minute
If DNA rotates at 600 rotations per minute, As each turn of helix has 10 base pairs, it will be relaxing 6000 base pairs as required for the replication fork to proceed.
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Show and explain the "Arithmetic" behind the following statement: During eukaryotic DNA replication, when the replication...
multiple answers are possible! Which of the following statement about DNA is FALSE? When bacteriophage DNA...
multiple answers are possible!
Which of the following statement about DNA is FALSE? When bacteriophage DNA was shown to be the agent necessary for production of new phages, this was the early experiment demonstrating that DNA is the hereditary material. During DNA replication, two replication forks are formed to complete the synthesis of the entire chromosome in eukaryotes. During bacterial DNA replication process, replication forks move in opposite directions away from the origin of replication The molecular structure of DNA...
Which of the following statements about eukaryotic DNA replication is false? A) DNA polymerase can add...
Which of the following statements about eukaryotic DNA replication is false? A) DNA polymerase can add new nucleotides to either free 3'- or 5'-end B) RNA primers must be removed and rejected by the deoxynucleotides C) RNA primers must be laid down during DNA replication for synthesis of new DNA that starts at the 3'-OH of the primer ends D) Elongation of the DNA strand during replication requires primers with free 3'OH end E) The very end of the chromosome...
The speed of DNA replication at a replication fork is about 100 nucleotides per second per...
The speed of DNA replication at a replication fork is about 100 nucleotides per second per standin human cells. What is the minimum number of origins of replication that a human cell must have in order to replicate its DNA once every 24 hours? Recall that a human cell contains two copies of the human genome, one inherited from the mother and one from the father, each consisting of 3 x 109base pairs.
Which of the following is true regarding DNA replication (see Chapter 16 in the textbook). Check...
Which of the following is true regarding DNA replication (see Chapter 16 in the textbook). Check all that are correct. Question options: A) DNA ligase joins Okazaki fragments on the lagging strand. B) DNA replication results in one double helix with all parental strand and one double helix with completely newly synthesized DNA. C) Nucleases add RNA primers to start replication. D) DNA replication starts at multiple points along the eukaryotic genome. E) Helicases prevent separation of the DNA strands...
List other types of errors that occur during replication of DNA.7. Explain why the formation of m...
List other types of errors that occur during replication of DNA.7. Explain why the formation of mismatched base pairs during replication can cause a mutation.
A) Explain lagging strand DNA replication in detail. Underline the following terms in your answer: replication...
A) Explain lagging strand DNA replication in detail. Underline the following terms in your answer: replication fork, DNA polymerase III, primase, and ligation. Make sure that your answer is complete and that all the entities that come together in the process of lagging strand replication are clearly explained. Draw one figure of a replication fork with the polarity (directionality) of each DNA strand indicated. G) Explain RNA transcription in E. coli in detail, from initiation to termination. Underline the following...
Complete each sentence to explain DNA replication. three parent strand as the During DNA replication, the...
Complete each sentence to explain DNA replication.
three parent strand as the During DNA replication, the double-stranded DNA bonds connecting the parent strands are broken. hydrogen covalent An enzyme called is responsible for this step. DNA polymerase helicase New position themselves along the parent strands through nucleotides two The nucleotides are joined to each oth by an enzyme called complementary base pairing unwinds Now, new daughter DNA molecules are produced, each consisting of one old and one new rendering DNA...
During both DNA replication and PCR the DNA double helix must be unwound. Explain how this...
During both DNA replication and PCR the DNA double helix must be unwound. Explain how this is achieved during each process.
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open...
In the following diagram, A and B are two Okazaki fragments
generated during DNA replication. Open boxes represent the primers
and dotted lines represent the newly synthesized DNA strands.
a. To which direction (left or right) is the replication fork is
moving? (1 mark)
b. Which Okazaki fragment is made first in the diagram? Explain.
(2 marks)
c. (i) Which enzyme in E. coli synthesizes the primers on
Okazaki fragments? (1 mark)
(ii) What is the difference of this enzyme...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open boxes represent the primers and dotted lines represent the newly synthesized DNA strands. B ---- Template DNA a. To which direction (left or right) is the replication fork is moving? (1 mark) b. Which Okazaki fragment is made first in the diagram? Explain. (2 marks) c. (i) Which enzyme in E. coli synthesizes the primers on Okazaki fragments ? (1 mark) (ii) What is...
multiple answers are possible!
Which of the following statement about DNA is FALSE? When bacteriophage DNA was shown to be the agent necessary for production of new phages, this was the early experiment demonstrating that DNA is the hereditary material. During DNA replication, two replication forks are formed to complete the synthesis of the entire chromosome in eukaryotes. During bacterial DNA replication process, replication forks move in opposite directions away from the origin of replication The molecular structure of DNA...
Complete each sentence to explain DNA replication.
three parent strand as the During DNA replication, the double-stranded DNA bonds connecting the parent strands are broken. hydrogen covalent An enzyme called is responsible for this step. DNA polymerase helicase New position themselves along the parent strands through nucleotides two The nucleotides are joined to each oth by an enzyme called complementary base pairing unwinds Now, new daughter DNA molecules are produced, each consisting of one old and one new rendering DNA...
In the following diagram, A and B are two Okazaki fragments
generated during DNA replication. Open boxes represent the primers
and dotted lines represent the newly synthesized DNA strands.
a. To which direction (left or right) is the replication fork is
moving? (1 mark)
b. Which Okazaki fragment is made first in the diagram? Explain.
(2 marks)
c. (i) Which enzyme in E. coli synthesizes the primers on
Okazaki fragments? (1 mark)
(ii) What is the difference of this enzyme...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open boxes represent the primers and dotted lines represent the newly synthesized DNA strands. B ---- Template DNA a. To which direction (left or right) is the replication fork is moving? (1 mark) b. Which Okazaki fragment is made first in the diagram? Explain. (2 marks) c. (i) Which enzyme in E. coli synthesizes the primers on Okazaki fragments ? (1 mark) (ii) What is...
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