What is ubiquitin ligase? A) The enzyme that transfers ubiquitin onto the 20S subunit from the...
What is ubiquitin ligase?
A) The enzyme that transfers ubiquitin onto the 20S subunit from the protein destined for degradation.
B) The enzyme that removes ubiquitin from proteins in the proteasome.
C) The enzyme that transfers ubiquitin onto proteins that are destined for degradation.
Homework Answers
Answer: (C)
Explanation: Ubiquitination, a post-translational modification of proteins requires the sequential action of three enzymes. E1, or ubiquitin-activating enzyme, catalyzes the ATP-dependent activation of ubiquitin. Ubiquitin is then transferred to a E2 (ubiquitin-conjugating enzymes) and through the E3 (ubiquitin ligase) to the protein substrate destined for degradation by the proteasome.
E3 (ubiquitin ligase) recognize a substrate through specific short amino acid sequences, known as degrons, and catalyze the transfer of ubiquitin from the E2 to the protein substrate.
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What is ubiquitin ligase?
A) The enzyme that transfers ubiquitin onto the 20S subunit from
the...
Phosphorylation-dependent binding of F-box protein B-TrCP containing E3 ubiquitin ligase to human WEE1A. S123 is phosphorylated...
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please answer all parts
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Part A The sigma (?) subunit binds to the core RNA polymerase enzyme. The function of...
Part
A
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to the promoter of a gene so that transcription can be initiated.
The closeup shows the secondary structure of the sigma (?)
subunit, which consists of four domains. Identify the domains
labeled 1-3.
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to...
pls fo all 20) A) an enzyme that synthesizes RNA as part of the transcription process...
pls fo all
20) A) an enzyme that synthesizes RNA as part of the transcription process B) an enzyme that uses RNA as a substrate C) an enzyme that catalyzes the association between the large and small ribosomal subunits D) an enzyme that synthesizes RNA primers during DNA replication E) an RNA with enzymatic activity 20) What is a ribozyme? 21) 21) Alternative RN A splicing A) increases the rate of transcription. B) can allow the production of similar proteins...
Q 4. Explain how Fig.1A (arrow) result corroborates with the author hypothesis about Parkin aggre...
Q
4. Explain how Fig.1A (arrow) result corroborates with the author
hypothesis about Parkin aggresome and proteasome activity in PD?
MG-132+ HMW 127- 84-- 41- DTT+ IP T HMW 6- IP : anti-flag WB: anti-ubiquitin Fia. 1. Parkin forms plexes A, HEK 293Tells transiently tranafected with FLAG-Parkin were divided into twe dishes and were either untreated or treated with 5PM MG-132 for 161). Cells were then lysed abidใหr-taining 1% Triton X-100 ลnd fractitated into "Iuble (S) and insoluble u) frac...
Phosphorylation-dependent binding of F-box protein B-TrCP containing E3 ubiquitin ligase to human WEE1A. S123 is phosphorylated by a CDK and the phosphorylation primes CK2 to phosphorylate S121 resulting in creation of a B-TCP phosphodegron (EEGFGpS121) that is responsible for the instability of WEE1A during interphase. At the onset of M-phase, when activated Plk1 accumulates, Plk1 binds to WEE1A to the PBD binding motif surrounding PS123 (SpSP) via its PBD and phosphorylates S53 resulting in generation of the second phosphodegron (DpSAFQE)....
The GTPase Dynamin is involved in which step of vesicular A) Binding of cargo molecules to cargo receptors B) Assembly of the Clathrin coat C) Pinching off the vesicle from the membrane D) Transport of vesicles along microtubules Which of the following events does not occur in the rough endoplasmic reticulum? A) Cleavage of the signal peptide by the signal peptidase B) Binding of chaperone proteins to misfolded or partially folded pathways C) Transfer of a pre formed oligosaccharide to...
please answer all parts
106. What process helps compact the DNA in a chromosome? A) anaphase promoting complex B) entry into S-phase of the cell cycle C) removal of the nuclear lamina D) removal of methyl groups from DNA E) histone de-acetylation 107. What does DNA Ligase do? A) removes mismatched nucleotides B) adds complimentary bases to single stranded DNA C) joins the ends of chromosomes together D) forms a phosphodiester covalent bond between adjacent nucleotides E) chews up single...
Part
A
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to the promoter of a gene so that transcription can be initiated.
The closeup shows the secondary structure of the sigma (?)
subunit, which consists of four domains. Identify the domains
labeled 1-3.
The sigma (?) subunit binds to the core RNA polymerase
enzyme. The function of this sigma factor is to recognize and bind
to...
pls fo all
20) A) an enzyme that synthesizes RNA as part of the transcription process B) an enzyme that uses RNA as a substrate C) an enzyme that catalyzes the association between the large and small ribosomal subunits D) an enzyme that synthesizes RNA primers during DNA replication E) an RNA with enzymatic activity 20) What is a ribozyme? 21) 21) Alternative RN A splicing A) increases the rate of transcription. B) can allow the production of similar proteins...
Q
4. Explain how Fig.1A (arrow) result corroborates with the author
hypothesis about Parkin aggresome and proteasome activity in PD?
MG-132+ HMW 127- 84-- 41- DTT+ IP T HMW 6- IP : anti-flag WB: anti-ubiquitin Fia. 1. Parkin forms plexes A, HEK 293Tells transiently tranafected with FLAG-Parkin were divided into twe dishes and were either untreated or treated with 5PM MG-132 for 161). Cells were then lysed abidใหr-taining 1% Triton X-100 ลnd fractitated into "Iuble (S) and insoluble u) frac...
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