Question

Part A

This image shows the RNA Polymerase holoenzyme space-fill model. What is the approximate location of the active site?

The sigma (?) subunit binds to the core RNA polymerase enzyme. The function of this sigma factor is to recognize and bind to the promoter of a gene so that transcription can be initiated. The closeup shows the secondary structure of the sigma (?) subunit, which consists of four domains. Identify the domains labeled 1-3.

This image shows the RNA Polymerase holoenzyme space-fill model. What is the approximate location of the active site?

The sigma (?) subunit binds to the core RNA polymerase enzyme. The function of this sigma factor is to recognize and bind to the promoter of a gene so that transcription can be initiated. The closeup shows the secondary structure of the sigma (?) subunit, which consists of four domains. Identify the domains labeled 1-3.

	N-terminal domain 1 (1), N-terminal domain 2 (2), C-terminal domain (3)
	N-terminal domain 2 (1), N-terminal domain 1 (2), C-terminal domain (3)
	C-terminal domain (1), N-terminal domain 2 (2), N-terminal domain 1 (3)
	C-terminal domain (1), N-terminal domain 1 (2), N-terminal domain 2 (3)

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Part B

This image shows a space-filling model of the RNA polymerase holoenzyme for Thermus thermophilus. Each subunit is a different color. Identify the subunits labeled 1-3.

This image shows a space-filling model of the RNA polymerase holoenzyme for Thermus thermophilus. Each subunit is a different color. Identify the subunits labeled 1-3.

	Sigma (?) subunit C-terminal domain (1), sigma (?) subunit N-terminal domain 1 (2), beta (?) subunit (3)
	Sigma (?) subunit N-terminal domain 1 (1), sigma (?) subunit C-terminal domain (2), beta prime (?�) subunit (3)
	Sigma (?) subunit N-terminal domain 1 (1), sigma (?) subunit C-terminal domain (2), beta (?) subunit (3)
	Sigma (?) subunit C-terminal domain (1), sigma (?) subunit N-terminal domain 1 (2), beta prime (?�) subunit (3)

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Part C

Which two subunits of the RNA polymerase holoenzyme form a cleft that contains a deep channel down in which the DNA template binds?

Which two subunits of the RNA polymerase holoenzyme form a cleft that contains a deep channel down in which the DNA template binds?

	beta (?) and sigma (?) subunits
	sigma (?) and omega (?) subunits
	beta prime (?�) and beta (?) subunits
	sigma (?) and beta prime (?�) subunits

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Answer 1

Part A:

The correct option among the following is 3^rd one- C-terminal domain (1), N-terminal domain 2 (2), N-terminal domain 1 (3)

Part B:

The correct option among the following is 4^th one- Sigma subunit C-terminal domain (1), sigma subunit N-terminal domain 1 (2), beta prime subunit (3).

Part C:

The beta clamp forms the beta pincer, which undergoes large conformational change to clamp down around internal channel to hold DNA and RNA.

The correct option among the following is 3^rd one- beta prime and beta subunits.