You want to resolve structures that electron microscopy has indicated are 55 nm apart in the...
You want to resolve structures that electron microscopy has indicated are 55 nm apart in the XY dimension. Which type of light microscopy technique(s) should you use?
Homework Answers
Answer:
It is better to use Transmission electron microscopy (TEM)” or “scanning electron microscopy (SEM)” to resolve structures that electron microscopy has indicated are 55 nm apart in the XY dimension.
The resolution of Transmission electron microscopy (TEM) is about nearly 0.1 nm whereas resolution of SEM is about 2 nm.
The resolving power of a microscope: It is defined as the smallest distance that is apart followed by the wavelength of incident light on the sample associated with quality of lenses.
Rule of thumb, the resolution of the light microscope is 200nm in X or Y and in Z dimension it is about 700nm
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You want to resolve structures that electron microscopy has
indicated are 55 nm apart in the...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy [EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
To resolve details of a cell using an ordinary microscope, you must use a wavelength that...
To resolve details of a cell using an ordinary microscope, you must use a wavelength that is about the same size, or smaller, than the details of the cell you want to observe. Suppose you wamt to be able to see the ribosomes, which are about 22.0 nm in diameter. If you use an electron microscope, what is the minimum kinetic energy of the electrons? eV
Quantum confinement becomes important at small dimensions. In one dimension, quantum confinement ...
Quantum confinement becomes important at small dimensions. In one dimension, quantum confinement becomes important at Δx = (h^2/m*kBT)1/2 where m* is either the mass of the electron or mass of the hole. (a) Calculate the dimension at which quantum confinement becomes significant for n-type and p-type gallium arsenide at 300 K. (b) How many layers are needed for quantum confinement of electrons or holes? Information you might want to use: Gallium arsenide has an electron effective mass of 0.067 me...
You want to coat a pane of glass that has an index of refraction of 1.54...
You want to coat a pane of glass that has an index of refraction of 1.54 with a 195-nm-thick layer of material that has an index of refraction greater than that of the glass. The purpose of the coating is to cancel light reflected off the top of the film having a wavelength (in air) of 540 nm. The coating material is very expensive, so you want to use the thinnest possible layer. 1) What should be the index of...
Question 23 (1 point) A lesion on skin has been identified. You want to generate a...
Question 23 (1 point) A lesion on skin has been identified. You want to generate a photochemical reaction on this region. Assume that the lesion is concentrated with hematoporphyrin derivative (HPD). What is the best method for triggering the photochemical reaction in the lesion? Expose the tumour directly with an Nd:Yag laser emitting at 2 = 1um Insert diffusers into the lesion in order to scatter red light at 2 - 633 nm in all directions 13 Use an Argon...
Question 23 (1 point) A lesion on skin has been identified. You want to generate a...
Question 23 (1 point) A lesion on skin has been identified. You want to generate a photochemical reaction on this region. Assume that the lesion is concentrated with hematoporphyrin derivative (HPD). What is the best method for triggering the photochemical reaction in the lesion? Expose the tumour directly with an Nd:Yag laser emitting at 1 = lum O Insert diffusers into the lesion in order to scatter red light at X = 633 nm in all directions Use an Argon...
You are working with a 55 year old female, 5'6" tall, 173 lbs. You want to...
You are working with a 55 year old female, 5'6" tall, 173 lbs. You want to guide her to safely losing 1 lb per week. She has been previously sedentary, and so will only commit to jogging 20 minutes per day (which burns 176 kcal), 3 days per week. What will her daily caloric intake need to be? How many grams of each of the 3 macronutrients should she eat with that diet? i i B I T F wit.cunctionnaire
Please answer the questions 12 to 21 shown in the photo attachment accurately. Please. Thank you!...
Please answer the questions 12 to 21 shown in the photo
attachment accurately. Please. Thank you!
12. The following is incorrect regarding mitochondria and plastids: A) They originated by invagination rial DNA is homologous to that of ricketsias C) Chloroplasts originated from D) They have bacterial-type 70S ribosomes E) None of the above. 13. One of these techniques is mismatched with the specimen under study. Which one is i? on bacterial cell wall surfaces-Atomi force microscopy B) Stained bacterial cells-Bright-field...
) In the figure, a slit 0.30 mm wide is illuminated by light of wavelength 426 nm. A diffraction attern is seen on a screen 2.8 m from the slit. What is the linear distance on the screen betw...
) In the figure, a slit 0.30 mm wide is illuminated by light of wavelength 426 nm. A diffraction attern is seen on a screen 2.8 m from the slit. What is the linear distance on the screen between e first diffraction minima on either side of the central diffraction maximum? Answer: 8.0 mm 30) A thin beam of laser light of wavelength 514 nm passes through a diffraction grating having 3952 lines/cm. The resulting pattern is viewed on a...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy [EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
To resolve details of a cell using an ordinary microscope, you must use a wavelength that is about the same size, or smaller, than the details of the cell you want to observe. Suppose you wamt to be able to see the ribosomes, which are about 22.0 nm in diameter. If you use an electron microscope, what is the minimum kinetic energy of the electrons? eV
You want to coat a pane of glass that has an index of refraction of 1.54 with a 195-nm-thick layer of material that has an index of refraction greater than that of the glass. The purpose of the coating is to cancel light reflected off the top of the film having a wavelength (in air) of 540 nm. The coating material is very expensive, so you want to use the thinnest possible layer. 1) What should be the index of...
Question 23 (1 point) A lesion on skin has been identified. You want to generate a photochemical reaction on this region. Assume that the lesion is concentrated with hematoporphyrin derivative (HPD). What is the best method for triggering the photochemical reaction in the lesion? Expose the tumour directly with an Nd:Yag laser emitting at 2 = 1um Insert diffusers into the lesion in order to scatter red light at 2 - 633 nm in all directions 13 Use an Argon...
Question 23 (1 point) A lesion on skin has been identified. You want to generate a photochemical reaction on this region. Assume that the lesion is concentrated with hematoporphyrin derivative (HPD). What is the best method for triggering the photochemical reaction in the lesion? Expose the tumour directly with an Nd:Yag laser emitting at 1 = lum O Insert diffusers into the lesion in order to scatter red light at X = 633 nm in all directions Use an Argon...
You are working with a 55 year old female, 5'6" tall, 173 lbs. You want to guide her to safely losing 1 lb per week. She has been previously sedentary, and so will only commit to jogging 20 minutes per day (which burns 176 kcal), 3 days per week. What will her daily caloric intake need to be? How many grams of each of the 3 macronutrients should she eat with that diet? i i B I T F wit.cunctionnaire
Please answer the questions 12 to 21 shown in the photo
attachment accurately. Please. Thank you!
12. The following is incorrect regarding mitochondria and plastids: A) They originated by invagination rial DNA is homologous to that of ricketsias C) Chloroplasts originated from D) They have bacterial-type 70S ribosomes E) None of the above. 13. One of these techniques is mismatched with the specimen under study. Which one is i? on bacterial cell wall surfaces-Atomi force microscopy B) Stained bacterial cells-Bright-field...
) In the figure, a slit 0.30 mm wide is illuminated by light of wavelength 426 nm. A diffraction attern is seen on a screen 2.8 m from the slit. What is the linear distance on the screen between e first diffraction minima on either side of the central diffraction maximum? Answer: 8.0 mm 30) A thin beam of laser light of wavelength 514 nm passes through a diffraction grating having 3952 lines/cm. The resulting pattern is viewed on a...
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