Homework Answers
ANS. Protein 2 will elute first because it does not get bound to anything.
ATP is a negatively charged molecule with four (-)ly charged oxygen atoms. So more salt will be required to overcome those interactions. So a high salt wash shoule be mandatory.
Add Answer to:
32. A biochemist is separating two proteins using an ATP-Sepharose column. Protein 1 binds ATP, but...
The five proteins listed below were run on a cation exchange column at pH = 7...
The five proteins listed below were run on a cation exchange column at pH = 7 Cytochrome C pl = 10.7 Ribonuclease A pl = 9.5 Myoglobin pl = 7.4 Carbonic anhydrase pl = 6.0 Glucose oxidase pl = 4.2 Which of the following statements is TRUE? A) Glucose oxidase and carbonic anhydrase will be separated in the low salt wash b) Cytochrome C binds and then elutes first when the salt concentration is increased c) decreasing the pH will...
How do you elute proteins from an affinity column where the protein is bound to a...
How do you elute proteins from an affinity column where the protein is bound to a ligand which is attached to beads? Add a large amount of the free ligand that specficially binds the protein to compete for binding to the protein. Add SDS to denature the proteins. Add increasing amounts of salt to compete for ionic bonds with the protein. Wash the column with a large amount of buffer so that even the smallest molecules can filter through.
1. A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in...
1. A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in a solution. Only three other proteins (A, B, and C) are present. The proteins have the following properties: pl (isoelectric point) Size Mr Bind to DNA? protein A 7.4 protein B 3.8 protein C 7.9 protein X7.8 82,000 21,500 23,000 22,000 yes yes no yes What type of protein separation techniques might she use to protein X from the other proteins. Give a flow...
You have a mixture of three proteins: Protein X, MW 15 kDa, pI 4.5 Protein Y, MW 25 kDa, pI 8.5 Protein Z, MW 75 kDa,...
You have a mixture of three proteins: Protein X, MW 15 kDa, pI 4.5 Protein Y, MW 25 kDa, pI 8.5 Protein Z, MW 75 kDa, pl 5.0 2) You have a cation exchange (acidic) column, low salt (100 mM NaCl) and high salt (1 M NaCI) buffer at pH 7.0. If you loaded the above mixture of proteins on the column in low salt buffer, which proteins would most likely be retained on the column? If you loaded the...
1) You are faced with separating two different proteins with identical mass. Protein A is a...
1) You are faced with separating two different proteins with identical mass. Protein A is a rod-like molecule with a great deal of secondary srtucture and protein B is a globular protein that is roughly spherical. What methods could be used to separate these two proteins? (check all that apply). A. Ion-exchange chromatography B. Two-dimensional electrophoresis C. SDS PAGE D. Size exclusion chromatography E. Affinity chromatography
(3 points) A Student uses cation exchange chromatography to separate Protein 1 pi9.5) and Protein 2...
(3 points) A Student uses cation exchange chromatography to separate Protein 1 pi9.5) and Protein 2 pl 45). The column is equilibrated using a 20m phosphate buffer, pH 6.0 and then the sample containing Protein 1 and Protein 2 is applied to the column. After the sample is applied to the column, the column is first washed with a low salt buffer. Subsequently, the column is washed with a high salt buffer. The elution profile is shown below. Clearly indicate...
In this case, Foe and Kemp purified the ribozyme phosphofructokinase-1 C (PFK-1 C) from brain tissue....
In this case, Foe and Kemp purified the ribozyme phosphofructokinase-1 C (PFK-1 C) from brain tissue. There are three ribozymes of PFK-1 and they are designated A, B, and C. The A ribozyme (M_r = 84,000 dal) is found in the muscle and the brain; the B ribozyme (M_r = 80,000 dal) is found in the liver and the brain; and the C ribozyme (M_r = 86,000) is found in the brain. Because the brain contains all three ribozymes and...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
Investigators introduce two proteins into the membrane of artificial lipid vesicles: (1) an ATP synthase isolated...
Investigators introduce two proteins into the membrane of artificial lipid vesicles: (1) an ATP synthase isolated from the mitochondria of cow heart muscle, and (2) a light-activated proton pump purified from the prokaryote Halobacterium halobium. The proteins are oriented as shown in the diagram. When ADP and P; are added to the external medium and the vesicle is exposed to light, would this system produce ATP? H LIGHT protein pump ATP synthase Choose one: O No, because no electron-transport chain...
1. You have identified a new protein and predict it is a transmembrane protein due to...
1. You have identified a new protein and predict it is a transmembrane protein due to its AA sequence analysis. How would determine where the protein is located in the cell? A. Extract all the proteins from the cell and analyze them on a protein gel. B.Use Polymerase Chain Reaction (PCR) to amplify the protein. C. Use an fluorescent antibody to the protein and allow it to bind to the cells. D Isolate the RNA for the protein and use...
How do you elute proteins from an affinity column where the protein is bound to a ligand which is attached to beads? Add a large amount of the free ligand that specficially binds the protein to compete for binding to the protein. Add SDS to denature the proteins. Add increasing amounts of salt to compete for ionic bonds with the protein. Wash the column with a large amount of buffer so that even the smallest molecules can filter through.
1. A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in a solution. Only three other proteins (A, B, and C) are present. The proteins have the following properties: pl (isoelectric point) Size Mr Bind to DNA? protein A 7.4 protein B 3.8 protein C 7.9 protein X7.8 82,000 21,500 23,000 22,000 yes yes no yes What type of protein separation techniques might she use to protein X from the other proteins. Give a flow...
You have a mixture of three proteins: Protein X, MW 15 kDa, pI 4.5 Protein Y, MW 25 kDa, pI 8.5 Protein Z, MW 75 kDa, pl 5.0 2) You have a cation exchange (acidic) column, low salt (100 mM NaCl) and high salt (1 M NaCI) buffer at pH 7.0. If you loaded the above mixture of proteins on the column in low salt buffer, which proteins would most likely be retained on the column? If you loaded the...
(3 points) A Student uses cation exchange chromatography to separate Protein 1 pi9.5) and Protein 2 pl 45). The column is equilibrated using a 20m phosphate buffer, pH 6.0 and then the sample containing Protein 1 and Protein 2 is applied to the column. After the sample is applied to the column, the column is first washed with a low salt buffer. Subsequently, the column is washed with a high salt buffer. The elution profile is shown below. Clearly indicate...
In this case, Foe and Kemp purified the ribozyme phosphofructokinase-1 C (PFK-1 C) from brain tissue. There are three ribozymes of PFK-1 and they are designated A, B, and C. The A ribozyme (M_r = 84,000 dal) is found in the muscle and the brain; the B ribozyme (M_r = 80,000 dal) is found in the liver and the brain; and the C ribozyme (M_r = 86,000) is found in the brain. Because the brain contains all three ribozymes and...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
Investigators introduce two proteins into the membrane of artificial lipid vesicles: (1) an ATP synthase isolated from the mitochondria of cow heart muscle, and (2) a light-activated proton pump purified from the prokaryote Halobacterium halobium. The proteins are oriented as shown in the diagram. When ADP and P; are added to the external medium and the vesicle is exposed to light, would this system produce ATP? H LIGHT protein pump ATP synthase Choose one: O No, because no electron-transport chain...
Most questions answered within 3 hours.
-
Question 29 Which of the following is not needed in order to
prepare a statement of...
asked 1 minute ago -
iodination of vanillin
experiment
1. Briefly explain why the
iodination took place at the indicated position...
asked 16 minutes ago -
Hello, I have questions on Matlab. Let's say that I have an
equation. Signal x(t)= cos(2*pi*10*t)...
asked 12 minutes ago -
Fermentation carried out by year cells will produce
1. glucos
2. ethanol
3. phsophoglyceraldehyde
4. 36...
asked 12 minutes ago -
create a class in Java for a Plane
the class will include
String Data Fields for...
asked 14 minutes ago -
A jewellery material consists of 92.5 wt% of Au and 7.5 wt% of
Cu forming a...
asked 19 minutes ago -
A uniform magnetic field B=5.210 T is perpendicular to the plane
of the paper (into the...
asked 26 minutes ago -
A metal strip 8.80 cm long, 0.758 cm wide, and 0.594 mm thick
moves with constant...
asked 27 minutes ago -
The following 32-bit binary word written in hexadecimal format
represents a single RISC-V assembly instruction. What...
asked 30 minutes ago -
A
survey found that out of a random sample of 200 workers 168 said
that they...
asked 41 minutes ago -
Duck Dodgers hops in his spaceship and leaves the Earth at a
constant velocity of 0.6c...
asked 44 minutes ago -
Describe three
key HR functions that must be integrated into the performance
management system to support...
asked 52 minutes ago