Homework Answers
In affinity chromatography, the bound protein can be collected by using appropriate buffers. This buffers will change the the binding conditions of protein to ligand, and thus it can be eluted .
Option 4 is right.
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How do you elute proteins from an affinity column where the protein is bound to a...
e stwc snct 2pts. Caci 1. In order to elute Con A from the affinity beads,...
e stwc snct 2pts. Caci 1. In order to elute Con A from the affinity beads, (a) 1.0M NaCI was used, (b) HRP was used, (c) the sephadex beads were washed twice with NaCl, (d) glucose was used, (e) the elution buffer contained a carbohydrate binding protein. 2. A specific biological property is used to separates macromolecules in (a) affinity chromatography (b) TLC, (c) ion exchange chromatography, (d) electrophoresis, (e) protein assay 3. In order to separate proteins from lectins...
32. A biochemist is separating two proteins using an ATP-Sepharose column. Protein 1 binds ATP, but...
32. A biochemist is separating two proteins using an ATP-Sepharose column. Protein 1 binds ATP, but Protein 2 does not. Which protein elutes first? And what should she use to remove the protein bound to the column? A. Protein 1; low salt wash B. Protein 1; high salt wash C. Protein 2; low salt wash D. Protein 2; high salt wash
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity...
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity chromatography but also to ___________. A. overexpress the proteins in bacteria B. to reduce toxicity of the foreign proteins C. for proper folding and keeping the proteins soluble D. to trace the cytological location of the foreign proteins 15. You have constructed an insulin-GST fusion protein and expressed it in E. coli. You want to separate the recombinant...
You are dissatisfied with the purity of the final preparation of your HRP and decided to...
You are dissatisfied with the purity of the final preparation of
your HRP and decided to include ion-exchange (IEC) and
gel-exclusion chromatography (GEC) steps in your purification
protocol. From the literature, you found out the following info
about HRP enzyme:
Molecular weight: 40,000
Isoelectric point: 7.2
HRP is a very stable protein.
2: Design GEC purification experiment using the information in
the appendix. Address and justify the following
I. choice of GEC column
II. choice of the buffer (if you...
Cheach characteristic to the appropriate chromatography technique. Description (11 items) (Drag and drop into the...
Cheach characteristic to the appropriate chromatography technique. Description (11 items) (Drag and drop into the appropriate area below) Antibodies are sometimes used to bind target proteins in this chromatography Small proteins are retained due to a longer pathway through the column. Metal ions can be fixed to a resin and bind to specific sequences of amino acids such as histidine Uses a porous bead made of complex carbohydrates that allow proteins to go through or around Two proteins of the...
1.) Describe the goals of a Gel Filtration Chromotography Experiment??? 2.) Explain each key theoretical principle...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into a tool for biotechnology? a. It could possibly be turned into a viral vector against lung cancers b. Its promoters might be used to express genes in lung cells c. Its surface proteins could be used for new epitope tags d. All of the above QUESTION 2 Which of the following are applications of molecular assembly described in this course? a. It can be...
For part 1 of this lab) I collected a soil sample from my campus Part 2)...
For part 1 of this lab) I collected a soil sample from my campus
Part 2) Tested bacteria initial viability Part 3) DNA extraction
Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing
Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA
sequence data analysis (sent sample to another lab)
Directions for Part 3 DNA extraction are in the attached
image
QUESTIONS REGARDING PART 3 (DNA extraction)
1) What type of conclusions can be made from initially...
e stwc snct 2pts. Caci 1. In order to elute Con A from the affinity beads, (a) 1.0M NaCI was used, (b) HRP was used, (c) the sephadex beads were washed twice with NaCl, (d) glucose was used, (e) the elution buffer contained a carbohydrate binding protein. 2. A specific biological property is used to separates macromolecules in (a) affinity chromatography (b) TLC, (c) ion exchange chromatography, (d) electrophoresis, (e) protein assay 3. In order to separate proteins from lectins...
32. A biochemist is separating two proteins using an ATP-Sepharose column. Protein 1 binds ATP, but Protein 2 does not. Which protein elutes first? And what should she use to remove the protein bound to the column? A. Protein 1; low salt wash B. Protein 1; high salt wash C. Protein 2; low salt wash D. Protein 2; high salt wash
You are dissatisfied with the purity of the final preparation of
your HRP and decided to include ion-exchange (IEC) and
gel-exclusion chromatography (GEC) steps in your purification
protocol. From the literature, you found out the following info
about HRP enzyme:
Molecular weight: 40,000
Isoelectric point: 7.2
HRP is a very stable protein.
2: Design GEC purification experiment using the information in
the appendix. Address and justify the following
I. choice of GEC column
II. choice of the buffer (if you...
Cheach characteristic to the appropriate chromatography technique. Description (11 items) (Drag and drop into the appropriate area below) Antibodies are sometimes used to bind target proteins in this chromatography Small proteins are retained due to a longer pathway through the column. Metal ions can be fixed to a resin and bind to specific sequences of amino acids such as histidine Uses a porous bead made of complex carbohydrates that allow proteins to go through or around Two proteins of the...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
For part 1 of this lab) I collected a soil sample from my campus
Part 2) Tested bacteria initial viability Part 3) DNA extraction
Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing
Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA
sequence data analysis (sent sample to another lab)
Directions for Part 3 DNA extraction are in the attached
image
QUESTIONS REGARDING PART 3 (DNA extraction)
1) What type of conclusions can be made from initially...
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