Graph the migration of ladder fragments (y-axis) vs. log (base 10) Fragment size (#bp) of ladder bands. Use the resulting STRAIGHT LINE to calculate the size of the PCR product in #bp.
Homework Answers
The ladder migration indicates that it to be an 50bp DNA ladder/ marker. The first band indicates the value 50bp, and the remaining base pair bands as follows : 100bp, 150,200,350,400,500,600,750,800,950bp.
Now the remaing bands of the lane 2,3,4,5 nears 100, 6,7, resembles 400, 8 band is 250 and 500bp
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Graph the migration of ladder fragments (y-axis) vs. log (base
10) Fragment size (#bp) of ladder...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
Table 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA ...
HELP PLEASE! at least with some examples.
size of dna ladder- 1.8cm
We were unable to transcribe this imageTable 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF...
segments over the length L of the string, where the length of each vibrating segment equals...
segments over the length L of the string, where the length of each vibrating segment equals one-half wavelength. Use this fact to show that the fr of the allowed standing waves on this string are given by fn-nfi, where n 1,2,3, 4,5,... and fi is the fundamental frequency. In other words, derive an expression relating the nth harmonic to the fundamental frequency. Yo may use the fact that the wave velocity is the same for all modes. 1. For a...
We understand that your diagram will not be able to portray the bands to their exact base-pair si...
We understand that your diagram will not be able to portray the bands to their exact base-pair size, and that in some cases you may not be able to calculate an exact size for your bands. Just do your best in terms of positioning the bands in the lanes with respect to the standard markers, but please do not panic if the bands are a couple of mm away from perfect in your diagram. LABEL each band with its size...
can you please help me with number 2 and 3? thanks Problem 2. Consider a version...
can you please help me with number 2 and 3? thanks
Problem 2. Consider a version of question 9 from the first WebWork problem set and an attempt at solving it. Find and explain the 3 mistakes that were made. Use the line numbers to identify where they occurred. Question 9: If a (base 10) double-log plot displays a line through the points (-3, -2) and (5,5), find the relation between x and y. Solution attempt: First, a correct double-log...
this is whst i have so its mire spread out. im not sure what else to...
this is whst i have so its mire spread out. im not sure what
else to add.. whst should i be looking for?
koa AB Top of Resolvine 200 116 31 22 14 WellA: MW (Protein Ladder). Well B: Sample Protein 1. Well : Sample Protein 2. Dye front Activities: Based on the picture of the destained SDS-PAGE gel, complete the following Information: -Migration distance of dye front: mm. Molecular Weight Log (MW) Distance travelled Ri (Da) of protein (mm)...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
Please complete tables for forensic biology lab using the gel image as reference. If you could...
Please complete tables for forensic biology lab using the gel
image as reference.
If you could complete the whole table that would be much
appreciated, however I do not expect anyone to do that much work
for me. If you could just complete a few rows on each one and show
me how you got there, then I'm sure I could complete the rest of it
(I'm just confused on how to calculate the distance in cm)
My teacher gave...
The dependent variable is the position (y-axis) and the independent variable is the time (x-axis). This...
The dependent variable is the position (y-axis) and the independent variable is the time (x-axis). This is the measured position of a rock as it falls from the top of a very high cliff and obeys the equations y = 1/2gt2. Make sure you label the plot properly. Right click on a data point in the graph and add a “Trend line.” Select a polynomial of order 2. This is a parabolic equation and describes an object falling under constant...
2.3*) Graph the following observations of x and y on graph paper. X 12 3 4...
2.3*) Graph the following observations of x and y on graph paper. X 12 3 4 5 6 la 10 8 55 23 bole (a) Using a ruler, draw a line that fits through the data. Measure the slope and intercept of the line you have drawn. (b) Use formulas (2.7) and (2.8) to compute, using only a hand calculator, the least squares estimates of the slope and the intercept. Plot this line on your graph. (c) Obtain the sample...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
HELP PLEASE! at least with some examples.
size of dna ladder- 1.8cm
We were unable to transcribe this imageTable 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF...
segments over the length L of the string, where the length of each vibrating segment equals one-half wavelength. Use this fact to show that the fr of the allowed standing waves on this string are given by fn-nfi, where n 1,2,3, 4,5,... and fi is the fundamental frequency. In other words, derive an expression relating the nth harmonic to the fundamental frequency. Yo may use the fact that the wave velocity is the same for all modes. 1. For a...
We understand that your diagram will not be able to portray the bands to their exact base-pair size, and that in some cases you may not be able to calculate an exact size for your bands. Just do your best in terms of positioning the bands in the lanes with respect to the standard markers, but please do not panic if the bands are a couple of mm away from perfect in your diagram. LABEL each band with its size...
can you please help me with number 2 and 3? thanks
Problem 2. Consider a version of question 9 from the first WebWork problem set and an attempt at solving it. Find and explain the 3 mistakes that were made. Use the line numbers to identify where they occurred. Question 9: If a (base 10) double-log plot displays a line through the points (-3, -2) and (5,5), find the relation between x and y. Solution attempt: First, a correct double-log...
this is whst i have so its mire spread out. im not sure what
else to add.. whst should i be looking for?
koa AB Top of Resolvine 200 116 31 22 14 WellA: MW (Protein Ladder). Well B: Sample Protein 1. Well : Sample Protein 2. Dye front Activities: Based on the picture of the destained SDS-PAGE gel, complete the following Information: -Migration distance of dye front: mm. Molecular Weight Log (MW) Distance travelled Ri (Da) of protein (mm)...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
Please complete tables for forensic biology lab using the gel
image as reference.
If you could complete the whole table that would be much
appreciated, however I do not expect anyone to do that much work
for me. If you could just complete a few rows on each one and show
me how you got there, then I'm sure I could complete the rest of it
(I'm just confused on how to calculate the distance in cm)
My teacher gave...
2.3*) Graph the following observations of x and y on graph paper. X 12 3 4 5 6 la 10 8 55 23 bole (a) Using a ruler, draw a line that fits through the data. Measure the slope and intercept of the line you have drawn. (b) Use formulas (2.7) and (2.8) to compute, using only a hand calculator, the least squares estimates of the slope and the intercept. Plot this line on your graph. (c) Obtain the sample...
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