Homework Answers
The correct answer is (d) Tubulin is expressed in all cell lines tested.
Since there is a 51kb band in all the cell types, this suggests that it is expressed in all the cell types.
When a gene displays similar expression in all cells, they are called house keeping genes. They are trasnscribed at all times and are very essential for functioning of the cell.
Statements (a) and (c) is wrong since the antibody used should be similar and it is not necessary that it binds to different epitopes.
Statement (e) is incorrrect because as observed from the blot that all the bands are of similar size.
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Consider the following western blot for tubulin. Each lane represents a different cell line culture. What...
Consider the following western blot for tubulin. Each lane represents a different cell line culture. What...
Consider the following western blot for tubulin. Each lane represents a different cell line culture. What can be concluded from this blot? HepG2 Hela SVT2 A549 COS7 Jurkat MDCK PC12 MCF7 191 97- 64- 51 -B-Tubulin 39 28 19 Select one: a. 8 different primary antibodies must have been used to produce this blot b. Tubulins from different organisms vary in size c. The primary antibody used identified different epitopes present in each type of tubulin d. More than one...
Question 1 Not yet answered Consider the following western blot for tubulin. Each lane represents a...
Question 1 Not yet answered Consider the following western blot for tubulin. Each lane represents a different cell line culture. What can be concluded from this blot? Points out of 2.50 P Flag question 191 97 64- 51 -B-Tubulin 39 28 19- Select one: a. Tubulins from different organisms vary in size O b. More than one statement can be concluded from this western blot c. Tubulin is expressed in all cell lines tested O d. The primary antibody used...
A human cell has an abnormal sex chromosome constitution of XXY. Where could the non-disjunction event...
A human cell has an abnormal sex chromosome constitution of XXY. Where could the non-disjunction event have occurred? Select one: a. Meiosis I of the father b. Meiosis Il of the mother c. Meiosis I of the mother d. Meiosis Il of the father e. More than one of these choices could result in an XXY cell Consider the following western blot for tubulin. Each lane represents a different cell line culture. What can be concluded from this blot? HepG2...
a. What organisms were the tubulin and the cyclin primary antibodies raised in? b. What was the purpose of the tubulin c...
a. What organisms were the tubulin and the cyclin primary antibodies raised in? b. What was the purpose of the tubulin control? What other proteins might you have used instead of tubulin? The aim of this week’s experiment is to verify how M-cyclin is varying within the synchronised tobacco BY2 cell culture. Protein levels will be assays using a dot blot immunoassay in which a crude protein extract is coated onto a nitrocellulose membrane and probed with specific antibodies. This...
Question 12 parts a,b,c Q12. Using the information provided below on ELISA, answer the following questions...
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into...
QUESTION 1 Although SARS-CoV-2 is currently a global health threat, how might we turn it into a tool for biotechnology? a. It could possibly be turned into a viral vector against lung cancers b. Its promoters might be used to express genes in lung cells c. Its surface proteins could be used for new epitope tags d. All of the above QUESTION 2 Which of the following are applications of molecular assembly described in this course? a. It can be...
Please summarise the following content:(no word limit) EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice,...
Please summarise the following content:(no word
limit)
EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permis...
explain figure 2 please on the third page
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permision of Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6550-63554, July 1992 Immunolog A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells RANDOLPH J. NOELLE时, MEENAKSHI ROY.. DAVID M. SHEPHERD., IVAN STAMENKOVICt JEFFREY A. LEDBETTER, AND ALEJANDRO ARUFFo Departmend Microbiology. Dartmouth Medical School One...
Which electrolyte measurement is least affected by hemolysis? a. Calciumb. b- Magnesium c. Potassium d. Total...
Which electrolyte measurement is least affected by hemolysis? a. Calciumb. b- Magnesium c. Potassium d. Total Bilirubin Question 2 of 35 Required Question Which of the following should cause specimen rejection for platelet aggregation studies? a. The sample hematocrit is too high .b. The sample is hemolyzed. c. The sample is over-anticoagulated. d. The sample platelet count is too low. Question 3 of 35 Required Question Which of the following is usually positive in a patient with a urinary tract...
BIOL 1620o, Spring 2019 NAME (Last, Preferred First): Date: 45. What is the most important means by which capillay exchange occurs? 53. Which of the following organs does NOT contain lymphatic...
BIOL 1620o, Spring 2019 NAME (Last, Preferred First): Date: 45. What is the most important means by which capillay exchange occurs? 53. Which of the following organs does NOT contain lymphatic tissue? A. liver B. spleen C. lingual tonsils A. exocytosis B. diffusiorn C. ATP powered pumps D. facilitated diffusion E. pharyngeal tonsils. 46.According to Poiseuille's law A. blood flow is not related to resistance. B. ph of the blood influences flow C. viscosity of the blood is not related...
Consider the following western blot for tubulin. Each lane represents a different cell line culture. What can be concluded from this blot? HepG2 Hela SVT2 A549 COS7 Jurkat MDCK PC12 MCF7 191 97- 64- 51 -B-Tubulin 39 28 19 Select one: a. 8 different primary antibodies must have been used to produce this blot b. Tubulins from different organisms vary in size c. The primary antibody used identified different epitopes present in each type of tubulin d. More than one...
Question 1 Not yet answered Consider the following western blot for tubulin. Each lane represents a different cell line culture. What can be concluded from this blot? Points out of 2.50 P Flag question 191 97 64- 51 -B-Tubulin 39 28 19- Select one: a. Tubulins from different organisms vary in size O b. More than one statement can be concluded from this western blot c. Tubulin is expressed in all cell lines tested O d. The primary antibody used...
A human cell has an abnormal sex chromosome constitution of XXY. Where could the non-disjunction event have occurred? Select one: a. Meiosis I of the father b. Meiosis Il of the mother c. Meiosis I of the mother d. Meiosis Il of the father e. More than one of these choices could result in an XXY cell Consider the following western blot for tubulin. Each lane represents a different cell line culture. What can be concluded from this blot? HepG2...
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
Please summarise the following content:(no word
limit)
EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...
explain figure 2 please on the third page
The Jounal of Immunol nes of the National Academy of Sciences USA 89. 6550-6554. 01992, by permision of Proc. Natl. Acad. Sci. USA Vol. 89, pp. 6550-63554, July 1992 Immunolog A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells RANDOLPH J. NOELLE时, MEENAKSHI ROY.. DAVID M. SHEPHERD., IVAN STAMENKOVICt JEFFREY A. LEDBETTER, AND ALEJANDRO ARUFFo Departmend Microbiology. Dartmouth Medical School One...
BIOL 1620o, Spring 2019 NAME (Last, Preferred First): Date: 45. What is the most important means by which capillay exchange occurs? 53. Which of the following organs does NOT contain lymphatic tissue? A. liver B. spleen C. lingual tonsils A. exocytosis B. diffusiorn C. ATP powered pumps D. facilitated diffusion E. pharyngeal tonsils. 46.According to Poiseuille's law A. blood flow is not related to resistance. B. ph of the blood influences flow C. viscosity of the blood is not related...
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