Question

Consider the following western blot for tubulin. Each lane represents a different cell line culture. What can be concluded from this blot? HepG2 Hela SVT2 A549 COS7 Jurkat MDCK PC12 MCF7 191 97- 64- 51 -B-Tubulin 39 28 19 Select one: a. 8 different primary antibodies must have been used to produce this blot b. Tubulins from different organisms vary in size c. The primary antibody used identified different epitopes present in each type of tubulin d. More than one statement can be concluded from this western blot e. Tubulin is expressed in all cell lines tested

Answer 1

CORRECT ANSWER: e.Tubulin is expressed in all cell lines tested.

Explanation: Tubulin is expressed in all cell lines because bands are visible in all lanes at same size (approx 50).

INCORRECT ANSWER:

a. 8 different primary antibodies must have been used to produce this blot

Explanation: Primary antibody binds to specific epitope or specific amino acid of protein of interest. In this case, tubulin has to be detected, so single primary antibody sepecific for tubulin could be use in order to detect each type of tubulin.

b. Tubulin from different organisms are different in size

Explanation: Tubulin isolated from different cell lines does not vary in size as indicated by single size band in all lanes.

c. The primary antibody used identified different epitopes present in each type of tubulin.

Explanation: Since single primary antibody can be used, this primary antibody usually detects only single epitope present in all tubulin. In other words, primary antibody identified single epitope present in each type of tubulin.

d. More than one statement can be concluded from this western blot.

Explanation: Here, most appropriate conclusion is option a.

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