Question

Question 1: As a researcher, you want to prevent gene expression by cleaving the product of transcription. Which regulatory mechanism would you use?

a, miRNA

b. siRNA

c. miRNA or siRNA

d. proteasome

e. RNA splicing

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Question 1a: Which statement represents a difference between DNA replication in your cells and amplification through PCR?

a. All of the above represents differences

b. DNA primers are required for PCR, but not in your cells

c. PCR involves differential temperature, but your cells do not

d. PCR makes two copies of DNA for every strand, but your cells make only a single copy

Will upvote if correct. Thank yuo

Answer 1

a isi- To prevent gene expression, I would use :- (c) miRNA or SiRNA. miRNA and siRNA are sregulatory RNAs that were discovered miRNA is transcribed as pairs with itself & folds over to make a hairpin that long RNA molecole, that base gets chopped up by enzymes and releasing a small double stranded fragment. One fragment is mature mirna that binds to a specific porotein & inhibits its expression. SiRNA is exogenous RNA that is taken up by cells, but miRNA is endogenous. Also, miRNAs can have multiple targets, but siRNA generally have specific target ya) The statement that represents a difference (C) PCR involves differential temperature, but fortsetten not in your cells. DNA replication in cells definitely requires primers; leading strand Synthesis requires single poimen, but a number of primers are needed strand synthesis which are latter removed and the Okazaki fragme ments generated are joined by cigase . DNA replication is semi-conservative and is catalyzed by DNA Polymerase and DNA is synthesized in 5' 3 d'orection PCR or Joolymerase chain reaction makes million of copies of specific region of DNA in vitro and is carried out by Tag Polymerase and requires forward and reverse primers. PCR has different temperatura requirement for three steps: -Denaturing - N966 ds DNA separates - Annealing- > Primers bind to complementary regions - Extension- ~ 72°C → Primers extended New DNA Strands Synthesized. for lagging ~55-65€