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The difference between PCR and real-time PCR is that real-time PCR: can determine the DNA lence, while standard PCR cannot. O
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1) Real time PCR as its name suggests can monitor the quantity of DNA for each replication cycle in real time. It does this by incorporating a fluorescent probe which attaches to DNA molecules. Therefore the amount of fluorescence will tell us the amount of DNA molecules. So we can measure the amplification while it happens in real time.

So the answer is - Option 3) It can measure the amount of DNA as the reaction proceeds while standard PCR cannot.

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