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In tandem mass spectrometry the unique second phase is characterized by running simultaneous samples to compare...

In tandem mass spectrometry the unique second phase is characterized by

running simultaneous samples to compare and confirm amino acid identity.

the further isolation of the first phase samples by two-dimensional gel electrophoresis.

the purification of the first phase samples by column chromatography.

ththe addition of a collision based fragmentation step to the first phase sample to determine amino acid sequence.e addition of a collision based fragmentation step to the first phase sample to determine amino acid sequence.

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Answer #1

1. In tandem mass spectrometry the unique second phase is characterized by

Tandem mass spectrometry used to produce structural information about a compound by further fragmenting the ions produced in the ion source.

so its unique second phase characterized by the addition of a collision based fragmentation step to the first phase sample to determine the amino acid sequence. so option D) is correct.

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