T/F DNA footprinting involves the enzyme DNaseI. What type of digest does one perform with DNaseI...
T/F
DNA footprinting involves the enzyme DNaseI. What type of digest does one perform with DNaseI in a footprinting experiment?
A. A complete digest
B. A limited digest
C. No digest because we use an inactive version of the DNAseI enzyme
T/F
All proteins that bind to specific DNA sites also bind to DNA nonspecifically.
A. TRUE
B. FALSE
T/F
Footprinting and EMSA assays can be used to purify sequence specific DNA binding proteins.
A. TRUE
B. FALSE
Homework Answers
1 Ans: The correct option is B (limited digestion).
DNA footprinting is performed to study DNA-protein interaction. It involves limited digestion as endonuclease cleaves DNA at the end bound to protein. Limited digestion yeilds fragmnet terminating everywhere except in footprint region.
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T/F
DNA footprinting involves the enzyme DNaseI. What type of digest
does one perform with DNaseI...
The observation that in any DNA sample, A T and G C A. DNase sequencing An...
The observation that in any DNA sample, A T and G C A. DNase sequencing An analytical method that determines which segments of DNA are bound by a particular B. Chargaff's rule protein factor, such as a transcription factor C. ChIP sequencing D. Euchromatin E. Histone acetylation F. major groove - # Areas associated with a eukaryotic gene that are where most DNA methylation occurs. # An analytical technique that involves a small slide or chip with many segments of...
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1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
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Muscle cells do not have the gene for keratin and...
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Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
thats all the information that he gave us to solve the question. Thank you for trying...
thats all the information that he gave us to solve the question.
Thank you for trying anyways
X C E Question 23 1 pts The free-energy changes for the transfer of individual amino acid residues from a hydrophobic to an aqueous environment are given as follows: Amino acid AG of transfer (kJ/mol Proline -0.8 -12.6 Histidine 6.7 Alanine Methionine 14.3 Based on this information, which of these amino acid pairs is MOST likely to be represented in membrane-spanning alpha helices?...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration;...
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The observation that in any DNA sample, A T and G C A. DNase sequencing An analytical method that determines which segments of DNA are bound by a particular B. Chargaff's rule protein factor, such as a transcription factor C. ChIP sequencing D. Euchromatin E. Histone acetylation F. major groove - # Areas associated with a eukaryotic gene that are where most DNA methylation occurs. # An analytical technique that involves a small slide or chip with many segments of...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
Question 2. True / False (10 marks) Tor F The DNA sequences shown below are complementary to each other. AATTGCTACGTT AATTGCTACGTT I or F T or F b. Primase carries polymerase activity. c. In prokaryotes, RNA polymerases synthesize RNA at a lower rate than DNA polymerases because it takes time for RNA polymerase to perform proofreading. d. Reverse transcriptase activity is observed in DNA polymerase I in prokaryotes. The base in the following nucleotide is adenine. This nucleotide can be...
42. Which statement is NOT true about DNA? a) DNA acts like an enzyme by binding amino acids together using dehydration synthesis. b) Before cells divide both strands must be copied and one is provided for each new cell cm RNA is copied from a piece of DNA termed a gene. d) DNA is not always copied perfectly. Mistakes, mutations, occur and result in changed proteins, e) DNA functions as code for the synthesis of proteins. 43. A section of...
Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
thats all the information that he gave us to solve the question.
Thank you for trying anyways
X C E Question 23 1 pts The free-energy changes for the transfer of individual amino acid residues from a hydrophobic to an aqueous environment are given as follows: Amino acid AG of transfer (kJ/mol Proline -0.8 -12.6 Histidine 6.7 Alanine Methionine 14.3 Based on this information, which of these amino acid pairs is MOST likely to be represented in membrane-spanning alpha helices?...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
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