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42. Based on some preliminary measurements, you suspect that a sample of enzyme contains an irreversible...
5. In this experiment, you made calculations beforehand to determine amounts needed in the procedure. Some...
5. In this experiment, you made calculations beforehand to determine amounts needed in the procedure. Some information was provided to help you make these calculations. Imagine that you are working in a laboratory and you need to find the concentration of an unknown sample of acetic acid, and you have no idea whatsoever what its concentration might be. What preliminary steps might you take to plan a titration that would accurately determine the concentration? (Consider: how might you decide what...
You are still interested in the enzyme happyase, which catalyzes the following reaction: HAPPY = SAD...
You are still interested in the enzyme happyase, which catalyzes the following reaction: HAPPY = SAD Previously, you determined that kcat = 400 s and Km = 10 M for your sample of happyase. Further research shows that this happyase sample was actually contaminated with a reversible inhibitor called ANGER. When ANGER is fully removed from the happyase preparation and [Eltotalis 4 nM, the measured Vmax is increased to 4.8 umes-1 and the measured Km is now 15 MM. Use...
2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
3. You did some research after the Enzyme lab and you found out that the optimal...
3. You did some research after the Enzyme lab and you found out that the optimal activity for the cellobiase enzyme was 40°C. In class, you conducted your experiment at room temperature, which is about 25°C. What would have happened to your experiment if you conducted it experiment inside a room at 5°C? What about if you conducted inside a room at 100°C? Hint: Tell me what will happen to your enzyme in each scenario AND if you expect to...
Help on prelab NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
Q1: Say you have a patient serum sample. You follow the Lab 8 kinetic enzyme assay...
Q1: Say you have a patient serum sample. You follow the Lab 8 kinetic enzyme assay procedure for lactate dehydrogenase (LD) and obtain a slope of 0.0212 min^-1 at 340 nm. What is the patient's LD activity? Please show a work. Q2: If you fall down a flight of stairs, your serum CK activity will rise for the next two days or so. Explain why the serum CK activity increases. Activity (U/L) = (12650)(rate) One U/L activity is defined as...
i Question. You are working for a Biotechnology company and with ghes you a tak to...
i Question. You are working for a Biotechnology company and with ghes you a tak to help create a viewem odcing a Eastoretreatshown in the action low. You have alre Rhod o dectes that performs the reaction so you decide to the whole cells n a n your d foran d the mu d You fit by the same name a s the difiche The With mixed luck from the wyme, you try using the whole Al shows the Where...
You work with a variety of apples that are red. Over the years, you've seen some...
You work with a variety of apples that are red. Over the years, you've seen some variants (mutants) show up among your plants, and decide to investigate this matter further. You come up with two true-breeding variants, one that gives yellow apples, and one that gives orange apples. Through a series of monohybrid crosses, you establish that both are caused by recessive alleles. And now you decide to see how those genes interact between them (if at all). But first...
YFG is a transcription factor that binds to the promoter of RIP, a gene that encodes...
YFG is a transcription factor that binds to the promoter of RIP, a gene that encodes for a protein that facilitates cellular secretion. YFG is required for RIP expression and reduction of RIP levels are linked to neuromuscular disorders, such as muscular dystrophy (MS). You discover 3 different point mutations in YFG in 3 different MS patients. You predict these mutations interfere with the stability of YFG. To test this, you create a luciferase reporter gene vector and transfect this...
??? DeepSea Explorer brings some sample near a hydrothermal vent on the seabed some 1000-meter deep....
???
DeepSea Explorer brings some sample near a hydrothermal vent on the seabed some 1000-meter deep. Upon examination with a light microscope, a unicellular star-shaped microorganism was discovered in the sample and cells were collected and their chemical compositions were analyzed. a) After analysis, scientists discover that these cells contain DNA, RNA, proteins and phospholipids Based on these results, you can conclude the organism is made of cells of Prokaryotic 1) Eukaryotic i) Cannot be determined b) Explain your reasoning...
5. In this experiment, you made calculations beforehand to determine amounts needed in the procedure. Some information was provided to help you make these calculations. Imagine that you are working in a laboratory and you need to find the concentration of an unknown sample of acetic acid, and you have no idea whatsoever what its concentration might be. What preliminary steps might you take to plan a titration that would accurately determine the concentration? (Consider: how might you decide what...
You are still interested in the enzyme happyase, which catalyzes the following reaction: HAPPY = SAD Previously, you determined that kcat = 400 s and Km = 10 M for your sample of happyase. Further research shows that this happyase sample was actually contaminated with a reversible inhibitor called ANGER. When ANGER is fully removed from the happyase preparation and [Eltotalis 4 nM, the measured Vmax is increased to 4.8 umes-1 and the measured Km is now 15 MM. Use...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
3. You did some research after the Enzyme lab and you found out that the optimal activity for the cellobiase enzyme was 40°C. In class, you conducted your experiment at room temperature, which is about 25°C. What would have happened to your experiment if you conducted it experiment inside a room at 5°C? What about if you conducted inside a room at 100°C? Hint: Tell me what will happen to your enzyme in each scenario AND if you expect to...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
i Question. You are working for a Biotechnology company and with ghes you a tak to help create a viewem odcing a Eastoretreatshown in the action low. You have alre Rhod o dectes that performs the reaction so you decide to the whole cells n a n your d foran d the mu d You fit by the same name a s the difiche The With mixed luck from the wyme, you try using the whole Al shows the Where...
???
DeepSea Explorer brings some sample near a hydrothermal vent on the seabed some 1000-meter deep. Upon examination with a light microscope, a unicellular star-shaped microorganism was discovered in the sample and cells were collected and their chemical compositions were analyzed. a) After analysis, scientists discover that these cells contain DNA, RNA, proteins and phospholipids Based on these results, you can conclude the organism is made of cells of Prokaryotic 1) Eukaryotic i) Cannot be determined b) Explain your reasoning...
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