Question

01. Transamination is a biochemical reaction by which the –NH2 group of one amino acid is transferred to another alpha-ketoacid for preparation of a new amino acid. This reaction is catalyzed by the transaminase, which uses _____ as a cofactor for formation of the catalytic intermediate _______ base.

Select one:

a. TPP ;;;; Lewis

b. TPP ;;;; Schiff

c. PLP ;;;; Lewis

d. PLP ;;;; Schiff

e. None of these

Answer 1

ANSWER

1- option D is correct.

• Transamination is a biochemical reaction by which the –NH2 group of one amino acid is transferred to another alpha-ketoacid for the preparation of new amino acids. This reaction is catalyzed by the transaminase, which uses PLP (Pyridoxal phosphate) as a cofactor for the formation of the catalytic intermediate Schiff base.
• Pyridoxal phosphate (PLP ) is an active form of vitamin B₆
• A Schiff base is a compound which is a sub-class of imines