Homework Answers
The genes will not be expressed in the 1st case because supersuppressor would mask their expression even in the presence of lactose.
In the 2nd case, the genes will not be expressed as there is no mention of presence of lactose. The suppressor is wildtype, and hence will mask the genes' expression.
In the 3rd case, the genes will be expressed as the operator has constitutive mutation.
In the 4th case, the genes will be expressed as lactose is present. Lactose inhibits the function of suppressor protein by binding to it.
In the 5th case, expression will occur because the constitutive mutation in operator is dominant to supersuppressor mutation.
Hence, expression of the genes will be seen in (3), (4), and (5).
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Question 20 4 pts MMC: For the lac operons below, choose the operons in which the...
MMC: For the lac operons below, choose the operons in which the genes (ZYA) would likely...
MMC: For the lac operons below, choose the operons in which the genes (ZYA) would likely be expressed. Terms: l'= no functional repressor made OC- operator does not bind repressor = the repressor does not bind the inducer P = does not bind the polymerase No superscript = wild type - O IPOZY A with high levels of glucose OP-OZYA O SPOCZYA with lactose OPOZY A without lactose IPOCZY A without lactose
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression...
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression of the normal (wild type) operon is given. Note that "ON" and "OFF" refer to whether the operon is expressed at high levels (ON) or basal//low/no levels (OFF). (Hint: mutations render the indicated site inactive while leaving the remaining part of the operon or protein functional.) Note...
What happens to the expression of the Lac gene of E. coli if the following conditions...
What happens to the expression of the Lac gene of E. coli if the following conditions occur? For each condition below, mark an X in the column labelled Activated or Repressed, then explain why you chose that answer. Please assume the glucose levels are low in the cell. The first row is done for you. Activated a Repressed b Explain your answer Repressor, Lactose, and RNA polymerase are all present in abundance X Repressor binds to lactose so it can...
Yet, all the cells in your body contain the same genes (and same alleles). The difference...
Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
gene regulation of bacteria operon systems. Protected View . Saved to this PG References Mailings Review...
gene regulation of bacteria operon systems. Protected View . Saved to this PG References Mailings Review View Help Tell me what you want to do ernet can contain viruses. Unless you need to edit, it's safe to stay in Protected View Enable Editing 5. What happens if lactose levels are low? Put the following list in order (1-5). RNA polymerase is blocked from transcribing the genes for the lactose metabolizing enzymes When RNA polymerase binds to the promoter, it cannot...
The diagram below illustrates the LAC operon in its OFF state when the inducer molecule —lactose—is...
The diagram below illustrates the LAC operon in its OFF state when the inducer molecule —lactose—is absent. Predict the ways in which the following conditions will affect the transcription of the lactose-utilization genes. OPERON Regulatory Promoter Operator_ gene Lactose-utilization genes DNA mRNA RNA polymerase cannot attach to promoter Active repressor Protein If a mutation in the regulatory gene results in a misfolding of the repressor protein so that it can no longer bind DNA, the lactose-utilization genes O Will be...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
The genotypes of the lac operon of several haploid and partial diploid E. coli strains are...
The genotypes of the lac operon of several haploid and
partial diploid E. coli strains are given. For each genotype,
indicate the status of B-galactosidase synthesis as either Yes or
No. Assume that no glucose is present and cAMP is rich in the
growth medium.
• Oc is a mutant operator that cannot be bound
by the wild type repressor protein.
• Is is a mutant of repressor gene and cannot
bind to operator
Haploid Partial Diploid IOZY IOCZY ISOZY...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
1) Which of the following conditions is most likely to cause the lactose operon to be...
1) Which of the following conditions is most likely to cause the lactose operon to be transcribed? A) There is more glucose in the cell than lactose. B) There is glucose but no lactose in the cell. C) The cyclic AMP and lactose levels are both high within the cell. D) The CAMP level is high and the lactose level is low. 2) How does the transcription of structural genes in an inducible operon occur? A) It occurs continuously in...
MMC: For the lac operons below, choose the operons in which the genes (ZYA) would likely be expressed. Terms: l'= no functional repressor made OC- operator does not bind repressor = the repressor does not bind the inducer P = does not bind the polymerase No superscript = wild type - O IPOZY A with high levels of glucose OP-OZYA O SPOCZYA with lactose OPOZY A without lactose IPOCZY A without lactose
For Questions 4-18, consider the mutations described in the left-hand column below. Identify whether the Lac operon will be ON or OFF in each of the environments listed across the top. The expression of the normal (wild type) operon is given. Note that "ON" and "OFF" refer to whether the operon is expressed at high levels (ON) or basal//low/no levels (OFF). (Hint: mutations render the indicated site inactive while leaving the remaining part of the operon or protein functional.) Note...
Yet, all the cells in your body contain the same
genes (and same alleles). The difference across cell types
is that genes get selectively expressed (turned on or off)
based on the proteins needed for cellular function given their
environment.
Select which statement explains the reason why hair does not
normally grow on your muscle cells.
a.
Muscle cells have the gene for keratin, but do not express
it
b.
Muscle cells do not have the gene for keratin and...
gene regulation of bacteria operon systems. Protected View . Saved to this PG References Mailings Review View Help Tell me what you want to do ernet can contain viruses. Unless you need to edit, it's safe to stay in Protected View Enable Editing 5. What happens if lactose levels are low? Put the following list in order (1-5). RNA polymerase is blocked from transcribing the genes for the lactose metabolizing enzymes When RNA polymerase binds to the promoter, it cannot...
The diagram below illustrates the LAC operon in its OFF state when the inducer molecule —lactose—is absent. Predict the ways in which the following conditions will affect the transcription of the lactose-utilization genes. OPERON Regulatory Promoter Operator_ gene Lactose-utilization genes DNA mRNA RNA polymerase cannot attach to promoter Active repressor Protein If a mutation in the regulatory gene results in a misfolding of the repressor protein so that it can no longer bind DNA, the lactose-utilization genes O Will be...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
The genotypes of the lac operon of several haploid and
partial diploid E. coli strains are given. For each genotype,
indicate the status of B-galactosidase synthesis as either Yes or
No. Assume that no glucose is present and cAMP is rich in the
growth medium.
• Oc is a mutant operator that cannot be bound
by the wild type repressor protein.
• Is is a mutant of repressor gene and cannot
bind to operator
Haploid Partial Diploid IOZY IOCZY ISOZY...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
1) Which of the following conditions is most likely to cause the lactose operon to be transcribed? A) There is more glucose in the cell than lactose. B) There is glucose but no lactose in the cell. C) The cyclic AMP and lactose levels are both high within the cell. D) The CAMP level is high and the lactose level is low. 2) How does the transcription of structural genes in an inducible operon occur? A) It occurs continuously in...
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