Question

1. What happens to the expression of the Lac gene of E. coli if the following conditions occur?
   For each condition below, mark an X in the column labelled Activated or Repressed, then explain why you chose that answer. Please assume the glucose levels are low in the cell. The first row is done for you.

   	Activated a	Repressed b	Explain your answer
   Repressor, Lactose, and RNA polymerase are all present in abundance	X		Repressor binds to lactose so it can no longer bind to the operator, RNA polymerase then actively transcribes the beta-galactosidase and lactose permease genes, and protein synthesis occurs.
   Repressor and RNA polymerase are both present in abundance, and there is no Lactose present
   Lactose and RNA polymerase are both present in abundance, and there is no Repressor present
   Lactose and Repressor are both present in abundance, and there is no RNA polymerase present
   RNA polymerase is present in abundance, and there is no Repressor protein or Lactose present

Answer 1

The solution has been done below:

Activared Repressed Reason ke prerror Lactose and RNA Polymer More are al pruent in abund one Lactose will act as inducer and bind to Ropressor, preventing it from binding with operator and switching 0-4 + ιαοορωπος. Repressora RNA polymert ase are both present in abundance and there is no lactose presenta Lac operon remain swirered off in absence of lachosen o Repressor is free, it will bind with operator, and no transcripHon will occure X Lactose and IRNA Polymer --are are both present in abundance and there is no repressor present. " Repressor is absent, operator is free, ea RNA Polymerase will bind and carry owl transcription Lactose and Repruson are both present in abundance and there is no RNA poly meron presente RNA polymerase is needed to carry our transcription If it is absent, no transcription. will occur, i Lal Operon will be switched off