Question

please answer 4 a and b

4. There were two main types of data that you obtained about your G protein and its associated mutations in cancer from a large cancer genomes database. Of the two main types of data (listed below), why are each of them important when considering G protein function, and what type of experiments were needed to obtain each type in the first place (this is not asking how you got them from the database, but how the original experiments were done)? (4 points, 2 points each) A. DNA sequencing of cancer genomes B. RNA sequencing from cancer cells

Answer 1

Answer 4a:)

DNA sequencing is important to identify SNPs in the genome. For this identification, the combination of Sanger sequencing and PCR is a powerful method. Other methods are whole-genome sequencing and structural variation studies in tumor genomes.

Answer 4b:)

RNA sequencing is useful for the quick analysis of somatic alterations in the mutants. Nowadays, next-generation sequencing of RNA extracted from tumor cells is a good tool to analyze complete transcriptome. This tool can identify allele-specific expression.

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