Question

Once an organism's genome sequence has been determined, which of the following methods is not used to identify all the genes within the genome?

Question options:

	a). Search for DNA sequences of genes known from other organisms
	b. Individually mutate nucleotides and determine which mutations cause a detectable phenotype
	c. Sequence cDNAs from different tissues and/or conditions and match them to the genome sequence.
	d. Look for open reading frames by searching for stretches of DNA sequence without stop codons.
	e. All of these methods are used to identify genes within a genome

Answer 1

All options are correct .

Closely related organism gene can be compared to find gene.

Mutation can result in affect of the function of the gene and thus phenotype which can indicate the gene function.

cDNA IS PREPARED FROM mRNA which is synthesized from gene and thus cDNA sequence can be compared to find genes.

Open reading frame with stop codon can be searched to find gene sequence of a organism after determining the genome sequence.

Thank you