Need help with this question. You are given a plasmid to map and you cut it...
Need help with this question.
You are given a plasmid to map and you cut it with Eco R1, Hind III and both. You produce the following bands
Eco R1 Hind III EcoR1/Hind III
2436 2810 1996
1989 2240 1275
1275 950 950
300 814
440
300
225
The plasmid is a closed circle. Using the data given produce a map with one of the Eco R1 sites at the zero point.
Eco R1 (0bp)
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Need help with this question.
You are given a plasmid to map and you cut it...
Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme...
Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme Eco R1. A map of the plasmid is shown here. The entire plasmid is 6000 bp, and there are Eco R1 restriction sites at 1500 bp, 2000 bp, and 4000 bp. You’re going to run the entire volume of the digest on a gel, and you want to cut just enough DNA to have 50 ng in the smallest band on your gel. Starting...
question number 7 purifying plasmid from your cells, you will set up a teaction to cut...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
Hi can someone help me understand part C and why the drawn in red lines are...
Hi can someone help me understand part C and why the
drawn in red lines are where they are.
Basically from the bp given how can I go back to cm so I can
drawn them into the picture provided?
Do not need help with A or B.
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes...
please help me with these questions Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping...
please help me with these questions
Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping the Plasmid The first step in mapping a plasmid is to determine how many times a restriction site is found on that plasmid. Examine the results for plasmid 55 as an example. The data given in the following table are for the double digest using EcoRI and Pstl. Also, giving are the data for single digests by the individual enzymes. The numbers in the...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
Hi can someone help me understand part C and why the
drawn in red lines are where they are.
Basically from the bp given how can I go back to cm so I can
drawn them into the picture provided?
Do not need help with A or B.
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes...
please help me with these questions
Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping the Plasmid The first step in mapping a plasmid is to determine how many times a restriction site is found on that plasmid. Examine the results for plasmid 55 as an example. The data given in the following table are for the double digest using EcoRI and Pstl. Also, giving are the data for single digests by the individual enzymes. The numbers in the...
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