Homework Answers
Number given side to restriction enzyme is restriction site from some reference point. Total length of plasmid is 5300bp.
a)For EcoR1 restriction sites are at 200 bp and 2700 bp.So,we will get two fragments.One of length 2500bp(2700-200=2500) and other of length 2800bp(5300-2500=2800).
b)For Hind3, restriction sites are at 1400bp and 3100bp from reference point.So,we will get two fragments.One of length 1700bp(3100-1400=1700) and other of length 3600bp(5300-1700=3600).
c)When we use combination of BamH1 and Hind2,we will get 4 restriction sites.Two for BamH1(one at 700 bp and other at 4500bp) and two for Hind3 (one at 1400bp other at 3100bp).So,we will get 4 fragments in total.
Lengths of fragments are 700bp(1400-700);1700bp(3100-1400);1400bp(4500-3100) and 1500bp[5300-(700+1700+1400)=1500].
d)Similarly,for combination of EcoR1 and BamH1 we will get 4 restriction sites.Restriction sites are at 200bp,700bp,2700bp and 4500bp from reference point.We will get 4 fragments.So length of each fragment is 500bp,2000bp,1800bp and 1000bp.
e) We will get a total of 6 restriction sites.Those are at 200,700,1400,2700,3100 and 4500 from reference point.So totally 6 fragments are generated.Lengths of 6 fragments are 500bp,700bp,1300bp,400bp,1400bp and 1000bp.
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7. Use the diagram of the plasmid below to answer the following questions 5,300 bp E...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis. Assume there is...
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
The following questions are based on the plasmid maps outlined below. GFP (700 bp) Plasmid B...
The following questions are based on the plasmid maps outlined below. GFP (700 bp) Plasmid B (5.7 kb) ECORI Kpnl BamHI - Xmal Plasmid A (4.2 kb) Ampicillin resistance Kanamycin resistance 15. Outline the strategy for how you would clone the GFP cassette from plasmid A into plasmid B? Please include descriptions of how you would select for potentially correct clones and verify the final construct. For the purposes of this exercise, assume that sequencing services are not available. *...
construct a restriction of a circular pvn plasmid, using the following data yor map should indicale,...
construct a restriction of a circular pvn plasmid, using the following data yor map should indicale, either the relative positions of each restriction site or give the distances between the restriction J DNA sizes of fragments ( bp) uncut DNA 112000 PNA cut with sma I 12000 DNA cut with Hind III 2000 PNA cut with ECORI 12000 onn cut with sma I FEcori 7000, 5000 ana cut with SMO I + HINDIII 10.000, 2000 min cut with Hind II...
QUESTION 11 a) The diagram below shows a typical plasmid cloning vector. There are three components ORI, amp and restri...
QUESTION 11 a) The diagram below shows a typical plasmid cloning vector. There are three components ORI, amp and restriction enzyme recognition sizes. Explain the roles of each of these three components and explain why each is important for cloning genes. 6 marks] ORI Hindlll Sphl Pstl Sall Restriction Sites Xbal BamHI Smal Kpnl Sac EcoRI amp ORI role: importance: amp role: importance: Restriction sites role: importance: b) A technique called RT-PCR uses the enzyme reverse transcriptase (RT) in combination...
Use the following depiction of a gel of Hind III fragments to answer the questions: 1...
Use the following depiction of a gel of Hind III fragments to answer the questions: 1 Kb Ladder Possible Hind III Fragments: 1 Kb Ladder Sizes 10,000 8,000 564 bp 8,888 2.0 kb 2.3 kb 11 4.36 kb 4,000 3,000 2,500 2,000 1,500 6.5 kb 9.4 kb 23 kb 1,000 750 500 250 1. Based on the gel results, list the sizes of the chromosome fragments cloned: 2. Based on the gel results, list the sizes of the chromosome fragments...
please answer question numbe 2. Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
The extracted plasmid DNA is pUC19. Look up the plasmid map and include in your pre-lab...
The extracted plasmid DNA is pUC19. Look up the plasmid map and
include in your pre-lab as well as your report.
a. What is the location of the BamH1 restriction site? Xmn1
restriction site? (sequence location by number). How many of these
sites exist each?
b. If single digestions were performed (one restriction enzyme)
with BamH1 and Xmn1, respectively, how many fragments would form,
and what would be the sizes of each of these fragments?
c. If a double digestion...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
The following questions are based on the plasmid maps outlined below. GFP (700 bp) Plasmid B (5.7 kb) ECORI Kpnl BamHI - Xmal Plasmid A (4.2 kb) Ampicillin resistance Kanamycin resistance 15. Outline the strategy for how you would clone the GFP cassette from plasmid A into plasmid B? Please include descriptions of how you would select for potentially correct clones and verify the final construct. For the purposes of this exercise, assume that sequencing services are not available. *...
construct a restriction of a circular pvn plasmid, using the following data yor map should indicale, either the relative positions of each restriction site or give the distances between the restriction J DNA sizes of fragments ( bp) uncut DNA 112000 PNA cut with sma I 12000 DNA cut with Hind III 2000 PNA cut with ECORI 12000 onn cut with sma I FEcori 7000, 5000 ana cut with SMO I + HINDIII 10.000, 2000 min cut with Hind II...
QUESTION 11 a) The diagram below shows a typical plasmid cloning vector. There are three components ORI, amp and restriction enzyme recognition sizes. Explain the roles of each of these three components and explain why each is important for cloning genes. 6 marks] ORI Hindlll Sphl Pstl Sall Restriction Sites Xbal BamHI Smal Kpnl Sac EcoRI amp ORI role: importance: amp role: importance: Restriction sites role: importance: b) A technique called RT-PCR uses the enzyme reverse transcriptase (RT) in combination...
Use the following depiction of a gel of Hind III fragments to answer the questions: 1 Kb Ladder Possible Hind III Fragments: 1 Kb Ladder Sizes 10,000 8,000 564 bp 8,888 2.0 kb 2.3 kb 11 4.36 kb 4,000 3,000 2,500 2,000 1,500 6.5 kb 9.4 kb 23 kb 1,000 750 500 250 1. Based on the gel results, list the sizes of the chromosome fragments cloned: 2. Based on the gel results, list the sizes of the chromosome fragments...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
The extracted plasmid DNA is pUC19. Look up the plasmid map and
include in your pre-lab as well as your report.
a. What is the location of the BamH1 restriction site? Xmn1
restriction site? (sequence location by number). How many of these
sites exist each?
b. If single digestions were performed (one restriction enzyme)
with BamH1 and Xmn1, respectively, how many fragments would form,
and what would be the sizes of each of these fragments?
c. If a double digestion...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
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