Question

in 6% NaCl test Vibrio cholera is positive what are the results, observation, and conclusion of this test?

Answer 1

During test procedure, the inoculum used in the test should be light enough to prevent visible turbidity before incubation of the broths. The broths are incubated at 35˚ to 37˚C and read at 18 to 24 hours. In the absence of overnight growth, they may be incubated for up to 7 days.

RESULT

V.cholerae shows positive in 6% NaCl test

OBSERVATION

А в с

V. cholerae grows in the absence of NaCl (tube B), but growth is stimulated by the addition of 1% NaCl (tube A). Tube C, 0% NaCl, inoculated with V. parahaemolyticus, shows no growth.

In the proposed experiment, the strain of V.cholerae is highly halophilic.

CONCLUSION

V cholerae grows well in different salt conditions is a well adapted halotolerant and halophilic bacteria.

If salinity is decreased, the optimum levels of growth will decreased. Most of the Vibrio family are salt lovers.

Higher salt concentrations will often slow down growth, but certainly not inactivate the organisms. If conditions become more favourable (lowering of salt concentration), they will come back.