Use the results of the agarose gel electrophoresis separation to
construct a restriction map for the sample of DNA
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Use the results of the agarose gel electrophoresis separation to
construct a restriction map for the...
3. The given figure represents the agarose gel electrophoresis results from a restriction digest experiment. Lane...
3. The given figure represents the agarose gel electrophoresis results from a restriction digest experiment. Lane 1 is a DNA ladder (values are in kb) and Lane 2 is the DNA sample cut by a restriction enzyme. What is the size of the band C in lane 2? - 1111111 About 1.8 kb Cannot tell based on the information provided 500 bp About 2 kb © Less than 1.5 bp -
A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis. Assume there is...
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA...
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA with the enzymes BamHI and HindIII separately and then load the fragments into an agarose gel and perform electrophoresis. Next, you perform a Southern analysis using the 4,878-bp EcoRI lambda fragment as a probe. a. Draw a picture of the electrophoresis gel, using the outline of the stained electrophoresis gel in Worksheet 16.IIIB (the two smallest HindIII fragments will run off the gel.) b....
Genetics Restriction Mapping A 7.0kb plasmid is cut at a Pstl site and a 4.0kb piece...
Genetics Restriction Mapping
A 7.0kb plasmid is cut at a Pstl site and a 4.0kb piece of DNA is inserted forming a recombinant plasmid. A restriction digest is conducted and then visualized with agarose gel electrophoresis. Construct a plasmid map of the recombinant fragment using the foliowing size information (in kb about the cuts produced by the digest. Map this plasmid. The way to present your answer is very specific, see example below. 7.0 Pstl 4.0 6.0 ECORI 5.0 8.9...
Can someone help me with this homework question on gel electrophoresis? Translocation and deletion 3. You...
Can someone help me with this homework question on gel
electrophoresis?
Translocation and deletion 3. You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in determining whether a patient has a mutation within a specific DNA region. You know that this DNA segment of the "normal" gene does not include an EcoRI restriction site; but the mutated DNA segment of the same gene contains an EcoRI restriction site due to a point mutation at...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
A restriction map lists the locations of DNA sequences that are cut by a particular restriction...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
Module 2 homework Laurel Jacqmai 0341 - Spring 20-MEEK > Activities and Due Dates > Module...
Module 2 homework Laurel Jacqmai 0341 - Spring 20-MEEK > Activities and Due Dates > Module 2 homework Assignment Score: 20% Resources Hint Check Ans < Question 2 of 5 > EcoRI Control EcoRI BamHI BamHI Base pairs 10 kb Samples of a plasmid containing a segment of unknown DNA are digested using the restriction enzymes EcoRI, BamHI, and a combination of EcoRI and BamHI. The digests are then run on an agarose gel in order to separate the resulting...
3. The given figure represents the agarose gel electrophoresis results from a restriction digest experiment. Lane 1 is a DNA ladder (values are in kb) and Lane 2 is the DNA sample cut by a restriction enzyme. What is the size of the band C in lane 2? - 1111111 About 1.8 kb Cannot tell based on the information provided 500 bp About 2 kb © Less than 1.5 bp -
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA with the enzymes BamHI and HindIII separately and then load the fragments into an agarose gel and perform electrophoresis. Next, you perform a Southern analysis using the 4,878-bp EcoRI lambda fragment as a probe. a. Draw a picture of the electrophoresis gel, using the outline of the stained electrophoresis gel in Worksheet 16.IIIB (the two smallest HindIII fragments will run off the gel.) b....
Genetics Restriction Mapping
A 7.0kb plasmid is cut at a Pstl site and a 4.0kb piece of DNA is inserted forming a recombinant plasmid. A restriction digest is conducted and then visualized with agarose gel electrophoresis. Construct a plasmid map of the recombinant fragment using the foliowing size information (in kb about the cuts produced by the digest. Map this plasmid. The way to present your answer is very specific, see example below. 7.0 Pstl 4.0 6.0 ECORI 5.0 8.9...
Can someone help me with this homework question on gel
electrophoresis?
Translocation and deletion 3. You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in determining whether a patient has a mutation within a specific DNA region. You know that this DNA segment of the "normal" gene does not include an EcoRI restriction site; but the mutated DNA segment of the same gene contains an EcoRI restriction site due to a point mutation at...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
Module 2 homework Laurel Jacqmai 0341 - Spring 20-MEEK > Activities and Due Dates > Module 2 homework Assignment Score: 20% Resources Hint Check Ans < Question 2 of 5 > EcoRI Control EcoRI BamHI BamHI Base pairs 10 kb Samples of a plasmid containing a segment of unknown DNA are digested using the restriction enzymes EcoRI, BamHI, and a combination of EcoRI and BamHI. The digests are then run on an agarose gel in order to separate the resulting...
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