Homework Answers
1. A smear is a dried preparation of bacterial cells on a glass slide.
2. When heating organisms adhere to the slide and it kills the organisms. Also organisms take up the smear more regularly.
Add Answer to:
Questions: 1. What is a smear? 1 pt 2. List 2 reasons for heat-fixing a smear....
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media)...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
Assume that you stain encapsulated Streptococcus by applying a simple stain, carbolfuchsin (red) and then counterstaining...
Assume that you stain encapsulated Streptococcus by applying a simple stain, carbolfuchsin (red) and then counterstaining with a negative stain nigrosin (black). Through the microscope you will see The capsule is stained (list color). The cells are stained The background was stained List the approximate size range of the following: Viruses Prokaryote cells Eukaryote cells Give two reasons for heat fixing a bacterial smear on a slide before staining. What is the reason that simple stains will dye bacterial cells?
all questions ASSESSMENT Critical Thinking and Learning Outcomes Review 1. What are the two purposes of...
all questions
ASSESSMENT Critical Thinking and Learning Outcomes Review 1. What are the two purposes of heat fixation? 2. What is the purpose of simple staining? 3. Why are basic dyes more successful in staining bacteria than acidic dyes? 4. Name three basic stains 5. Why is time an important factor in simple staining? 6. How would you define a properly prepared bacterial smear? 7. Why should you use an inoculating needle when making smears from solid media? An inoculating...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
Chapter 4(microbiology) 1. What are the shapes and arrangements of bacteria cells? 2. Why is it...
Chapter 4(microbiology) 1. What are the shapes and arrangements of bacteria cells? 2. Why is it important to be able to identify the shape and arrangement of an unknown bacterial sample? 3. Differentiate between eubacteria and archaebacteria. 4. What are the differences between eukaryotes and prokaryotes? 5. Why would a microbiologist care about those differences? Be specific. 6. What are the basic parts of a prokaryote? (the parts found in all bacteria) 7. How do the cell walls of Gram...
10 Smear Preparation A. Short-Answer Questions 1. How does smear preparation of cells from a liquid...
10 Smear Preparation A. Short-Answer Questions 1. How does smear preparation of cells from a liquid me medium? ar preparation of cells from a liquid medium differ from preparation of cells from a solid 2. Why is it important to limit the quantity of cells used to prepare a smear? 3. Describe the potential consequences of making a smear that is too thick. 4. For preparation of a smear on a slide, what is the purpose of heat fixation? What...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin,...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
MICROBIOLOGY: Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential...
MICROBIOLOGY:
Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...
1) What is the purpose for using stains? 2) Why is it necessary to make a...
1) What is the purpose for using stains? 2) Why is it necessary to make a heat -fixed smear and what are the disadvantages of test fixing? 3) what is the benefit to using immersion oil when viewing bacteria? 4) When preparing a heat-fixed smear, what are some differences between liquid and solid. media? 5) Once oil has been added to your slide , which objective (s) can be used? cannot be used?
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. Define acidic and basic dyes. What is the purpose of each? 2. What is the purpose of fixing a slide that is to be stained? 3. Why are specimens to be stained suspended in sterile saline or distilled water? 4. Which of the microscope objectives is most satisfactory for studying bacteria?...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
Assume that you stain encapsulated Streptococcus by applying a simple stain, carbolfuchsin (red) and then counterstaining with a negative stain nigrosin (black). Through the microscope you will see The capsule is stained (list color). The cells are stained The background was stained List the approximate size range of the following: Viruses Prokaryote cells Eukaryote cells Give two reasons for heat fixing a bacterial smear on a slide before staining. What is the reason that simple stains will dye bacterial cells?
all questions
ASSESSMENT Critical Thinking and Learning Outcomes Review 1. What are the two purposes of heat fixation? 2. What is the purpose of simple staining? 3. Why are basic dyes more successful in staining bacteria than acidic dyes? 4. Name three basic stains 5. Why is time an important factor in simple staining? 6. How would you define a properly prepared bacterial smear? 7. Why should you use an inoculating needle when making smears from solid media? An inoculating...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
10 Smear Preparation A. Short-Answer Questions 1. How does smear preparation of cells from a liquid me medium? ar preparation of cells from a liquid medium differ from preparation of cells from a solid 2. Why is it important to limit the quantity of cells used to prepare a smear? 3. Describe the potential consequences of making a smear that is too thick. 4. For preparation of a smear on a slide, what is the purpose of heat fixation? What...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
MICROBIOLOGY:
Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. Define acidic and basic dyes. What is the purpose of each? 2. What is the purpose of fixing a slide that is to be stained? 3. Why are specimens to be stained suspended in sterile saline or distilled water? 4. Which of the microscope objectives is most satisfactory for studying bacteria?...
Most questions answered within 3 hours.
-
You follow the lab procedure and at the end of Part 4C, you used
an average...
asked 9 minutes ago -
Write 250-450 words, about the following: • Analyze how a
servant leader in your organization or...
asked 12 minutes ago -
3. American and Japanese workers can each produce 4 cars a
year. An American worker can...
asked 15 minutes ago -
On October 1, 20X1, a company purchased a piece of land by
agreeing to pay the...
asked 28 minutes ago -
Jamie is doing a survey at her school about whether the students
feel the cafeteria food...
asked 33 minutes ago -
What are the roles of 3' and 5' untranslated regions in
mRNAs?
asked 34 minutes ago -
A domestic television receives antenna delivers a sky noise
power of -105 dBm to a matched...
asked 32 minutes ago -
What do you think are the chemical reactions involved in the
breathalyzer tests administered to potentially...
asked 48 minutes ago -
A random sample of 120 observations produces a mean of
38.2 from a population with a...
asked 55 minutes ago -
The average area of land burned by forest fires every year in
Canada is 2.5 million...
asked 57 minutes ago -
36. The basic difference between government bureaus and market
firms is that bureaus:
a. can...
asked 56 minutes ago -
java
- A recursive method findR() to search for a number in list
(ArrayList)
- A...
asked 1 hour ago