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Assume that you stain encapsulated Streptococcus by applying a simple stain, carbolfuchsin (red) and then counterstaining...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media)...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
1. There are other staining procedures beyond simple staining and differential staining. Examples include endospore, acid-fast,...
1. There are other staining procedures beyond simple staining and differential staining. Examples include endospore, acid-fast, capsule, and flagella stains. Please pick one staining technique to research and answer the following questions: • What is the theory behind the staining technique, what stains are used, how are they applied, what portion of the bacteria are they reacting with • How is this stain used to differentiate or identify bacterial cells or cell structures? • Describe the staining technique. List and...
MICROBIOLOGY: Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential...
MICROBIOLOGY:
Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. Define acidic and basic dyes. What is the purpose of each? 2. What is the purpose of fixing a slide that is to be stained? 3. Why are specimens to be stained suspended in sterile saline or distilled water? 4. Which of the microscope objectives is most satisfactory for studying bacteria?...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
Puzzles, I need one or two word answer for each question. Thank you An acidic dye...
Puzzles, I need one or two word answer for each question. Thank you An acidic dye will give a ___________ stain. Dye in which the color portion resides in the positive ion. A bacterial cell wall containing only 2-3 layers of peptidoglycan and surrounded by an outer membrane composed of phospholipids, lipopolysaccharide, lipoprotein, and proteins. (2 words) During starch hydrolysis, starch is broken down to __________. Secreted by bacteria into the surrounding environment in order to break down larger nutrient...
A sentence or two stating the hypothesis or hypotheses made. A sentence or two describing what...
A sentence or two stating the hypothesis or hypotheses
made.
A sentence or two describing what was done
A summary of the most important results
A sentence stating the major conclusion
EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
MICROBIOLOGY:
Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. Define acidic and basic dyes. What is the purpose of each? 2. What is the purpose of fixing a slide that is to be stained? 3. Why are specimens to be stained suspended in sterile saline or distilled water? 4. Which of the microscope objectives is most satisfactory for studying bacteria?...
A sentence or two stating the hypothesis or hypotheses
made.
A sentence or two describing what was done
A summary of the most important results
A sentence stating the major conclusion
EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...
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