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A student measures k for a certain blue dye to be 200.4 M1 at 560 nm....
Sb %T> 30, 34 2) How would a student make 500.0 mL of a o.05200 M...
Sb %T> 30, 34 2) How would a student make 500.0 mL of a o.05200 M solution of irond(II) using a stock solution that is 0.3180 M in iron(II)? Explain how this would be done. 0-3180 Viso-08 18レ 3) A student measures k for a certain blue dye to be 200.4 M' at 560 nm. What is the molarity of the dye in a solution with an absorbance of 0.118 at this wavelength?
1.2 1.0 0.8 0.6 0.4+ 0.2 0+ 300 350 400 450 500 550 600 650 700 Wavelength (nm) The absorption spectrum of a certai...
1.2 1.0 0.8 0.6 0.4+ 0.2 0+ 300 350 400 450 500 550 600 650 700 Wavelength (nm) The absorption spectrum of a certain red dye is shown above. If a student analyzing the same concentration of this dye neglected to wipe fingerprints off the cuvette before placing it in the spectrophotometer, how would the absorption curve be affected? Absorbance
Chemistry lab question! Help please! A student finds a solution of blue dye #2 (another food...
Chemistry lab question! Help please!
A student finds a solution of blue dye #2 (another food dye we are not using in this lab) in a 1.0 cm cuvette has a maximum absorbance of 0.572 at 613 nm. The molar extinction coefficient, E, of blue #2 at the same wavelength is 1.21 * 10^4 M^-1 cm^-1. Calculate the concentration of this solution. The same student as in question 3 brings the blue dye #2 solution to her friend Bob to...
In a variation on the "secret recipe" part of this lab, one student secretly mixed together...
In a variation on the "secret recipe" part of this lab, one student secretly mixed together two different colors of solution and gave the mixture to another student to analyze. The student placed the sample in the MicroLab spectrophotometer and obtained the following graph of absorbance vs. wavelength. absorbance 400 430 460 500 540 580 620 660 700 viol indg blue grn gr-yel yel org red red wavelength (nm) Which two colors had the first student secretly mixed together? I.e....
Hi I am im a biochemistry lab and we are doing an experiment titled: PCR Amplification...
Hi
I am im a biochemistry lab and we are doing an experiment titled:
PCR Amplification of LeuRS F C Terminal Domain. I am having
troubles figuring out the rest of my caluclations.
I already did the calculations for the first dilution set, I
was wondering how I would do the calculation for the second
dilution set.
Also I was wondering how I would determine the theoretical
values for my data. Is there a specific formula I can use?
If...
Part C. Quantitative Spectroscopy Wavelength(A) Absorbance Amax-10 Amax 10 Wavelength used for Analysis in Part C:...
Part C. Quantitative Spectroscopy Wavelength(A) Absorbance Amax-10 Amax 10 Wavelength used for Analysis in Part C: Solution Concentration Absorbance % Transmittance Standard 1 Standard 2 Standard 3 Standard 4 Unknown 0.20 M Show an example calculation obtaining the molarity of the standards. a. Copper Standard 2: Obtain additional 0.20 M CuSO4 solution from the tray. Pipet 10 mL of 0.20 M CuSO4 solution into a 50 mL Erlenmeyer flask. Pipet 10 mL of DI water into the same tube and...
How do I make a line of best fit (regression) with the following data? I am...
How do I make a line of best fit (regression) with the
following data? I am aware of how to make it in Microsoft Excel,
but I am having trouble figuring out how to find my x value for
making the graph. Y value is absorbance, what do I use for x value
to calculate the slope?
Tube 0.5 mM sulfide standard (ml) Sulfide amount (umol) Sulfide reagent (ml) 0.0 4.9 0.11 0.00 0.2 FeCl3 dH2O to Absorbance 10ml total...
Sb %T> 30, 34 2) How would a student make 500.0 mL of a o.05200 M solution of irond(II) using a stock solution that is 0.3180 M in iron(II)? Explain how this would be done. 0-3180 Viso-08 18レ 3) A student measures k for a certain blue dye to be 200.4 M' at 560 nm. What is the molarity of the dye in a solution with an absorbance of 0.118 at this wavelength?
1.2 1.0 0.8 0.6 0.4+ 0.2 0+ 300 350 400 450 500 550 600 650 700 Wavelength (nm) The absorption spectrum of a certain red dye is shown above. If a student analyzing the same concentration of this dye neglected to wipe fingerprints off the cuvette before placing it in the spectrophotometer, how would the absorption curve be affected? Absorbance
Chemistry lab question! Help please!
A student finds a solution of blue dye #2 (another food dye we are not using in this lab) in a 1.0 cm cuvette has a maximum absorbance of 0.572 at 613 nm. The molar extinction coefficient, E, of blue #2 at the same wavelength is 1.21 * 10^4 M^-1 cm^-1. Calculate the concentration of this solution. The same student as in question 3 brings the blue dye #2 solution to her friend Bob to...
In a variation on the "secret recipe" part of this lab, one student secretly mixed together two different colors of solution and gave the mixture to another student to analyze. The student placed the sample in the MicroLab spectrophotometer and obtained the following graph of absorbance vs. wavelength. absorbance 400 430 460 500 540 580 620 660 700 viol indg blue grn gr-yel yel org red red wavelength (nm) Which two colors had the first student secretly mixed together? I.e....
Hi
I am im a biochemistry lab and we are doing an experiment titled:
PCR Amplification of LeuRS F C Terminal Domain. I am having
troubles figuring out the rest of my caluclations.
I already did the calculations for the first dilution set, I
was wondering how I would do the calculation for the second
dilution set.
Also I was wondering how I would determine the theoretical
values for my data. Is there a specific formula I can use?
If...
Part C. Quantitative Spectroscopy Wavelength(A) Absorbance Amax-10 Amax 10 Wavelength used for Analysis in Part C: Solution Concentration Absorbance % Transmittance Standard 1 Standard 2 Standard 3 Standard 4 Unknown 0.20 M Show an example calculation obtaining the molarity of the standards. a. Copper Standard 2: Obtain additional 0.20 M CuSO4 solution from the tray. Pipet 10 mL of 0.20 M CuSO4 solution into a 50 mL Erlenmeyer flask. Pipet 10 mL of DI water into the same tube and...
How do I make a line of best fit (regression) with the
following data? I am aware of how to make it in Microsoft Excel,
but I am having trouble figuring out how to find my x value for
making the graph. Y value is absorbance, what do I use for x value
to calculate the slope?
Tube 0.5 mM sulfide standard (ml) Sulfide amount (umol) Sulfide reagent (ml) 0.0 4.9 0.11 0.00 0.2 FeCl3 dH2O to Absorbance 10ml total...
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