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(kb) 1 2 4 5 6 7 8 15.5 14 9.5 5/4.5 Figure 2 above shows a series of restriction digests of a circular plasmid. lane e( S en BamHI EcoRI HindIII SalI BamHI and EcoRI BamHI and HindIII BamHI and Sall EcoRI and Sall 76. Referring to figure 2, if the plasmid is cut with EcoRI and HindIII, which size fragment is NOT a possibility? A) 0.5 B 1 C 7 D) 7.5 E) 9

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Answer #1

The plasmid is cut by EcoRI to obtain 14.5kb fragment. HindIII cuts the plasmid to produce 15.5kb fragment. if both EcoRI and HindIII cut the plasmid 1Kb fragment is to be obtained. There is no possibility to obtain 0.5Kb fragment. Therefore, option A is correct.

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