In microbiology, dilutions are critical for getting a countable number of microbes to grow on a...
In microbiology, dilutions are critical for getting a countable number of microbes to grow on a petri dish. If you suspect your culture of bacteria has 100 x 106 cells per mL, what would you want the final dilution to be in order to end up with 100 colonies of bacteria on a petri dish? Express your answer as an exponent rounded to two decimal places. Eg. 10.01e-3, not 0.00101. Treat colonies of bacteria (cells / mL) the way you would treat concentration (Moles / L) The formula M1V1 = M2V2 can still be used. The final concentration is the number of colonies on the petri dish. Solve for V2.
Homework Answers
CFU/ml = number of colonies / dilution factor * volume plated.
Given that CFU/ml =100 x 106 cells per mL
Number of colonies = 100
Suppose 1 ml of culture was plated.
100 x 106= 100 / Dilution factor
Dilution factor = 100/100*106= 10-6
So we need to dilute the culture by 10-6 times to get 100 colonies on the agar plate.
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In microbiology, dilutions are critical for getting a countable
number of microbes to grow on a...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100,...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
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I'm not sure if I completed question 3 correctly. Also, I need help determing question 4....
I'm
not sure if I completed question 3 correctly. Also, I need help
determing question 4.
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your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate 5th plate 6th plate 75 Which plate has the countable number of colonies? What is the dilution factor that produced that plate? • What is the correction factor for the volume? How many cfu/ml are in the culture, based on your calculations? 4. Suppose you plated 1 ml of a 10-7 dilution (tenfold dilutions) of a bacterial broth culture
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2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
I'm
not sure if I completed question 3 correctly. Also, I need help
determing question 4.
Y-0.301 6.51-hardts.al 3. A solution containing 100 mL of bacterial broth is subcultured onto 5 plates using the pour-plate method. Each plate receives broth according to the following Table. The Table also lists the results colony counts after 24 hours of incubation. Calculate the number of bacteria per milliliter of the bacterial broth for each of the dilutions. Plate Volume Broth Colonies 331 2...
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