Homework Answers
1) The picture with lowest MIC (Minimum Inhibitory concentration) is :-
(d) The zone of inhibition is Maximum.
Minimum inhibitory concentration is the lowest concentration of the antimicrobial agent that inhibit visible growth of the microorganism after overnight incubation.
In the figure (d) it has max area of inhibition that means the microorganism (bacteria) is most sensitive to this antibiotic. So it will have the lowest MIC value.
MIC value is inversely proportional to the zone of inhibition. More the zone of inhibition less will be MIC and vice versa. So the correct answer is (d).
2) The picture showing most effective antibiotic is :-
(d) Where the zone of inhibition is Maximum.
Effectiveness of an antibiotic is directly proportional to the zone of inhibition. Since in picture (d) there is maximum zone of inhibition i.e. bacteria are more sensitive to this antibiotic, so it will be the most effective.
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Below are images depicting the results of the Kirby-Bauer disk test. The same microbe was tested...
2. The MIC, Kirby Bauer and E-TEST are all tests that are used to determine which...
2. The MIC, Kirby Bauer and E-TEST are all tests that are used to determine which antibiotics work against specific bacteria. a. Your patient comes in with a Staphylococcus aureus infection and you know that tetracycline kills S. aureus, but you want to prescribe the patient with the lowest concentration of tetracycline, which test would you conduct to find the lowest concentration that killed off S. aureus? b. Your patient comes in with a mystery bacterial infection. You want to...
(2 points) Klebsiella is tested by disk diffusion assay (Kirby-Bauer test). A reference table is shown...
(2 points) Klebsiella is tested by disk diffusion assay (Kirby-Bauer test). A reference table is shown below to interpret the zones of inhibition. Antibiotic Resistant in mm Intermediate(mm) Susceptible (mm) Cefotaxime 11 12-13 ________ A. What would the minimum zone diameter be in this test that would indicate the organism is susceptible? ___________ B. If a tech used too high of an inoculum, for example used a solution of 10 million bacteria per ml instead of 100,000 per...
1. How can you prove that antibiotic used in Kirby-Bauer (disk diffusion) test is bactericidal? How...
1. How can you prove that antibiotic used in Kirby-Bauer (disk diffusion) test is bactericidal? How would you explain the presence of isolated colonies within an otherwise clear zone of inhibition produced in Kirby-Bauer (disk diffusion) test?
klebsiella is tested by disk diffusion assay kirky bauer test. Last edit was made seconds ago...
klebsiella is tested by disk diffusion assay kirky bauer test.
Last edit was made seconds ago by Maur wiew Insert Format Tools Add-ons Help is 3 4 2 1 100% - Normal text Arial - 11 - BI U AA 5 17. (2 points) Klebsiella is tested by disk diffusion assay (Kirby-Bauer test). A reference table is shown below to interpret the zones of inhibition Antibiotic Resistant in mm Intermediate(mm) Susceptible (mm) Cefotaxime 11 12-13 A. What would the minimum...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
Q11. when performing the Kirby-Bauer antibiotic test, the proper way to inoculate the bacteria on the...
Q11. when performing the Kirby-Bauer antibiotic test, the proper way to inoculate the bacteria on the agar plates is to (choose one) a. use a sterile loop and streak for isolated colonies b. use a sterile loop and inoculate using a zig-zag pattern on the plate c. use a sterile swab and streak for isolated colonies. d. use a sterile swab and cover the entire surface of the plate with the organism, creating a lawn of growth Q3. The term...
can you please help me with Qs 1-6 thanks ! LAB EXERCISE #6: Kirby-Bauer Disk Diffusion...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
1. Interpret the following standard antibiotic test results using the interpretive chart below. Assume that the...
1. Interpret the following standard antibiotic test results using the interpretive chart below. Assume that the method was used correctly and that all antibiotic discs were effective (not expired): (5 marks) a. Aeromonas hydrophilia tested with Tetracycline had an average zone size of 16.5 mm b. Aeromonas hydrophilia tested with Pencillin G had an average zone size of 10 mm c. Staphylococcus aureus Patient AB tested with Pencillin G had an average zone size of 24 mm. d. Staphylococcus aureus...
16 Name: Section Lab Report Date Observations and Results Part A: Kirby-Bauer Antibiotic Sensitivity Test Procedure...
16 Name: Section Lab Report Date Observations and Results Part A: Kirby-Bauer Antibiotic Sensitivity Test Procedure 1. Record the zone size and the susceptibility of each test organism to the chemotherapeutic agent as resistant (R), intermediate (1), or sensitive (S) in the charts below. E coli P. vulgaris GRAM-NEGATIVE Paeruginosa Zone Size Susceptibility ACID-FAST M. smegmatis Zone Size Susceptibility Chemotherapeutic Zone Size Zone Size Agent Susceptibility Susceptibility Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Sulfanilamide 32 32 GRAM-POSITIVE E. faecalis S....
2. The MIC, Kirby Bauer and E-TEST are all tests that are used to determine which antibiotics work against specific bacteria. a. Your patient comes in with a Staphylococcus aureus infection and you know that tetracycline kills S. aureus, but you want to prescribe the patient with the lowest concentration of tetracycline, which test would you conduct to find the lowest concentration that killed off S. aureus? b. Your patient comes in with a mystery bacterial infection. You want to...
klebsiella is tested by disk diffusion assay kirky bauer test.
Last edit was made seconds ago by Maur wiew Insert Format Tools Add-ons Help is 3 4 2 1 100% - Normal text Arial - 11 - BI U AA 5 17. (2 points) Klebsiella is tested by disk diffusion assay (Kirby-Bauer test). A reference table is shown below to interpret the zones of inhibition Antibiotic Resistant in mm Intermediate(mm) Susceptible (mm) Cefotaxime 11 12-13 A. What would the minimum...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
1. Interpret the following standard antibiotic test results using the interpretive chart below. Assume that the method was used correctly and that all antibiotic discs were effective (not expired): (5 marks) a. Aeromonas hydrophilia tested with Tetracycline had an average zone size of 16.5 mm b. Aeromonas hydrophilia tested with Pencillin G had an average zone size of 10 mm c. Staphylococcus aureus Patient AB tested with Pencillin G had an average zone size of 24 mm. d. Staphylococcus aureus...
16 Name: Section Lab Report Date Observations and Results Part A: Kirby-Bauer Antibiotic Sensitivity Test Procedure 1. Record the zone size and the susceptibility of each test organism to the chemotherapeutic agent as resistant (R), intermediate (1), or sensitive (S) in the charts below. E coli P. vulgaris GRAM-NEGATIVE Paeruginosa Zone Size Susceptibility ACID-FAST M. smegmatis Zone Size Susceptibility Chemotherapeutic Zone Size Zone Size Agent Susceptibility Susceptibility Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Sulfanilamide 32 32 GRAM-POSITIVE E. faecalis S....
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