When looking at the results of the negative control on the agarose gel, what do you...
When looking at the results of the negative control on the agarose gel, what do you expect to see?
Select one:
a. The products will be the same size as the blue and brown eye colour samples but the bands visible will be more faint (lighter).
b. You will see fewer bands when compared to the blue and brown eye colour samples.
c. There should be no PCR product visible.
d. You will see a PCR product, but it will be larger in size than the blue and brown eye colour samples.
Homework Answers
I believe the correct answer to be:
Option C) There should be no PCR product viile.
As the negative control contains no DNA or proteins and is used to verify that there are no previous contamination in the reaction
feel free to leave a comment down below for any further query. good rating would be appreciated if you find my answer helpful. thank you.
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When looking at the results of the negative control on the
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After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
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