Question

When looking at the results of the negative control on the agarose gel, what do you...

When looking at the results of the negative control on the agarose gel, what do you expect to see?

Select one:

a. The products will be the same size as the blue and brown eye colour samples but the bands visible will be more faint (lighter).

b. You will see fewer bands when compared to the blue and brown eye colour samples.

c. There should be no PCR product visible.

d. You will see a PCR product, but it will be larger in size than the blue and brown eye colour samples.

0 0
Add a comment Improve this question Transcribed image text
Answer #1

I believe the correct answer to be:

Option C) There should be no PCR product viile.

As the negative control contains no DNA or proteins and is used to verify that there are no previous contamination in the reaction

feel free to leave a comment down below for any further query. good rating would be appreciated if you find my answer helpful. thank you.

Add a comment
Know the answer?
Add Answer to:
When looking at the results of the negative control on the agarose gel, what do you...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • After PCR is performed the products are run out on an agarose gel. In the figure...

    After PCR is performed the products are run out on an agarose gel. In the figure below, grey bands represent the wells the PCR product was loaded into. The white bands represent DNA fragments produced by PCR. The target fragment amplified by the primers was 1,500 bp in size. The ladder is a standard DNA ladder containing bands of various sizes between 5,000 and 1,000 bp. The negative control contained only molecular grade water*. The positive control contained DNA known...

  • Explain at least one possible reason why you might have the following results in an agarose...

    Explain at least one possible reason why you might have the following results in an agarose gel electrophoresis of your PCR products. In addition to your DNA PCR sample, you also ran a Hi-Lo Marker, a positive PCR control, and a negative PCR control. 16. Scenario C: Your Hi-Lo marker showed up but there were no bands in the lanes for your PCR positive control, DNA PCR sample or negative PCR control.

  • Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for...

    Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...

  • 1. you have performed PCR and run your samples on a gel. When you look at...

    1. you have performed PCR and run your samples on a gel. When you look at the gel, you see very bright bands of DNA- what is true about this experiment A) there is ethidium bromide in the gel B) the PCR machine was not working C) you "ran to red" (samples run towards the positive electrode) D) you "ran to black" and ran your samples off the gel E)all of the above are reasons for the bright bands 2....

  • rate for answering questions compeletly. You plan to use a PCR based assay to determine whether...

    rate for answering questions compeletly. You plan to use a PCR based assay to determine whether 3 mosquitoes that you have collected are infected with West Nile virus. You have available to you three different virus-specific primers. The primers recognize sequences at the following nucleotide positions on the viral genome: primer 1: 155-176 primer 2: 745-720 primer 3: 926-905 The 5' and 3' ends of the genome are indicated on the long line The first nucleotide at the 5' end...

  • Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...

    Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel electrophoresis, the DNA fragments can be separated on a gel, based on their lengths. In order to see the fragments, a stain is typically added to the gel. The size of each fragment can be determined by comparing each one to a DNA molecular weight marker of known size. Below is a map of pBR22 plasmid. The position and base pair number of the...

  • Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...

    Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the horitzontal. I need help figuring out where to start and what to do. Please help! The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...

  • En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...

    En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....

  • Please I need help on questions 1-4 in great detail please Load 15 mu l of...

    Please I need help on questions 1-4 in great detail please Load 15 mu l of the following samples from the above section onto the simple Wells. Seal the wells with agarose and electrophorese until the bromophenol blue in the samples has migrated to within 2 mm of the positive electrode end of the gel. Remove the gels from the unit and stain them as described in Section IV. Measure the distance of the DNA bands (in cm) from the...

  • I need the answers for questions 2 and 3. My DNA ladder is in lane 2...

    I need the answers for questions 2 and 3. My DNA ladder is in lane 2 marked by the yellow arrow. Thanks! Here is the only other info I have. Thanks! Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT