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60. The number of tRNAs required for synthesis of proteins in E. coli is a 32...
51. E. coli is being used to study various agents that inhibit protein synthesis. One agent binds...
51. E. coli is being used to study various agents that inhibit protein synthesis. One agent binds the 23S rRNA of the 50S subunit and prevents peptide bond formation. No new proteins are produced. To which antibiotic is this agent likely related? a. chloramphenicol b. streptomycin c. puromycin d. tetracycline e. erythromycin We were unable to transcribe this image57. A protein that affects the expression of many different genes is known as a L 74. One benefit of operons in...
7. In bacteria the elongation stage of protein synthesis does not involve: A) aminoacyl-tRNAS B) EF-Tu...
7. In bacteria the elongation stage of protein synthesis does not involve: A) aminoacyl-tRNAS B) EF-Tu C) GTP D) IF-2 E) peptidyl transferase Which of the following best describes the cholesterol molecule? A) Amphipathic B) Nonpolar, charged C) Nonpolar, uncharged D) Polar, charged E) Polar, uncharged The enzyme that attaches an amino acid to a tRNA (aminoacyl-tRNA synthetase): A) always recognizes only one specific tRNA. B) attaches a specific amino acid to any available tRNA species. C) attaches the amino...
O) The role of the 23S TRNA of the large ribosomal subunit of E. coli during...
O) The role of the 23S TRNA of the large ribosomal subunit of E. coli during protein synthesis is: a. aid in initiation of protein synthesis b. contributes to the process of translocation during the elongation stage of protein synthesis c. helps to form the A site within the ribosome d. actively forms a peptide bond e. none of the above P) Spontaneous mutations can be caused by: a. incorrect translation b. telomerase c. chemical mutagens d. tautomeric shifts e....
The FepC, D and G are proteins of E. coli are required to transport Fe-siderophore complexes...
The FepC, D and G are proteins of E. coli are required to transport Fe-siderophore complexes from the periplasm across the cytoplasmic membrane into the cytoplasm. In order to first transport these complexes from the environment into the periplasm, the outer membrane protein FepA is required. Expression of the fepA gene is regulated in the same way as expression of the genes in the fepCDG operon. Given this information, which one of the following statements is correct? A. In the...
QUESTION 34 The gene encoding an E. coli tRNA containing the anticodon 5'-GUA-3' mutates so that...
QUESTION 34 The gene encoding an E. coli tRNA containing the anticodon 5'-GUA-3' mutates so that the anticodon now is 5'-UUA-3'. What will be the effect of this mutation? a. The bacterial ribosomes would stop translation whenever a tyrosine codon in a mRNA is positioned in the A site. b. The bacterial ribosomes would stop translation whenever a leucine codon in a mRNA is positioned in the A site. c. The bacterial ribosomes would compete with the termination factor to...
DNA, Genes and Protein Synthesis Activity 13: Protein Synthesis is the process by which cells produce (synthesize) proteins. An overview of the process is shown in model 2 (below). Gone 2...
DNA, Genes and Protein Synthesis Activity 13: Protein Synthesis is the process by which cells produce (synthesize) proteins. An overview of the process is shown in model 2 (below). Gone 2 Gene 1 Gene 3 DNA strand3 TRANSLATION Protein Trp Gly Model 2 ACTIVITY and QUESTIONS 1. Based on the information you can gather from model 1 complete the following sentences: a. The nucleotide Adenine (A) always pairs with the nucleotide b. The nucleotide Guanine (G) always pairs with the...
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair...
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair sequence called • The initiation of DNA replication at this site begins with the binding of the protein, to multiple 9 base pair repeats regions. The association of these proteins generate superhelical tension which unwinds the 13 bp repeat region called the . The next step of initiation involves the loading of the protein (a helicase) onto the 13 bp repeat region by another...
want to double check! 25. Th e DNA sequences encoding the initiation whese parated bcoding the...
want to double check!
25. Th e DNA sequences encoding the initiation whese parated bcoding the initiation and termination codons of a certain protein amino acids in length. there of the protei nucleotides on a certain organism's chromosome; however ssuming that there has been no post-translational processing elined from this gene is translated, the protein product is only 250 A. The RNA was synthesized in a bacterial cell n, what can you conclude from these observations? The RNA was synthesized...
25. What binds to a stop codon on a mRNA during translation? a. transcription factor c....
25. What binds to a stop codon on a mRNA during translation? a. transcription factor c. termination factor b. tRNA d. transcription initiator 26. What is typically attached to the acceptor end of a tRNA? a. a protein b. an amino acid C a ribosome d. a nucleosome 27. During mRNA processing, what is put on the 3' end of a primary mRNA transcript? a. a poly-A tail b. a cap d. an intron c. an exon 28. Which of...
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity...
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity chromatography but also to ___________. A. overexpress the proteins in bacteria B. to reduce toxicity of the foreign proteins C. for proper folding and keeping the proteins soluble D. to trace the cytological location of the foreign proteins 15. You have constructed an insulin-GST fusion protein and expressed it in E. coli. You want to separate the recombinant...
51. E. coli is being used to study various agents that inhibit protein synthesis. One agent binds the 23S rRNA of the 50S subunit and prevents peptide bond formation. No new proteins are produced. To which antibiotic is this agent likely related? a. chloramphenicol b. streptomycin c. puromycin d. tetracycline e. erythromycin We were unable to transcribe this image57. A protein that affects the expression of many different genes is known as a L 74. One benefit of operons in...
7. In bacteria the elongation stage of protein synthesis does not involve: A) aminoacyl-tRNAS B) EF-Tu C) GTP D) IF-2 E) peptidyl transferase Which of the following best describes the cholesterol molecule? A) Amphipathic B) Nonpolar, charged C) Nonpolar, uncharged D) Polar, charged E) Polar, uncharged The enzyme that attaches an amino acid to a tRNA (aminoacyl-tRNA synthetase): A) always recognizes only one specific tRNA. B) attaches a specific amino acid to any available tRNA species. C) attaches the amino...
O) The role of the 23S TRNA of the large ribosomal subunit of E. coli during protein synthesis is: a. aid in initiation of protein synthesis b. contributes to the process of translocation during the elongation stage of protein synthesis c. helps to form the A site within the ribosome d. actively forms a peptide bond e. none of the above P) Spontaneous mutations can be caused by: a. incorrect translation b. telomerase c. chemical mutagens d. tautomeric shifts e....
QUESTION 34 The gene encoding an E. coli tRNA containing the anticodon 5'-GUA-3' mutates so that the anticodon now is 5'-UUA-3'. What will be the effect of this mutation? a. The bacterial ribosomes would stop translation whenever a tyrosine codon in a mRNA is positioned in the A site. b. The bacterial ribosomes would stop translation whenever a leucine codon in a mRNA is positioned in the A site. c. The bacterial ribosomes would compete with the termination factor to...
DNA, Genes and Protein Synthesis Activity 13: Protein Synthesis is the process by which cells produce (synthesize) proteins. An overview of the process is shown in model 2 (below). Gone 2 Gene 1 Gene 3 DNA strand3 TRANSLATION Protein Trp Gly Model 2 ACTIVITY and QUESTIONS 1. Based on the information you can gather from model 1 complete the following sentences: a. The nucleotide Adenine (A) always pairs with the nucleotide b. The nucleotide Guanine (G) always pairs with the...
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair sequence called • The initiation of DNA replication at this site begins with the binding of the protein, to multiple 9 base pair repeats regions. The association of these proteins generate superhelical tension which unwinds the 13 bp repeat region called the . The next step of initiation involves the loading of the protein (a helicase) onto the 13 bp repeat region by another...
want to double check!
25. Th e DNA sequences encoding the initiation whese parated bcoding the initiation and termination codons of a certain protein amino acids in length. there of the protei nucleotides on a certain organism's chromosome; however ssuming that there has been no post-translational processing elined from this gene is translated, the protein product is only 250 A. The RNA was synthesized in a bacterial cell n, what can you conclude from these observations? The RNA was synthesized...
25. What binds to a stop codon on a mRNA during translation? a. transcription factor c. termination factor b. tRNA d. transcription initiator 26. What is typically attached to the acceptor end of a tRNA? a. a protein b. an amino acid C a ribosome d. a nucleosome 27. During mRNA processing, what is put on the 3' end of a primary mRNA transcript? a. a poly-A tail b. a cap d. an intron c. an exon 28. Which of...
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