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1: CRISPR technology is used to alter gene expression. i) In addition to a target DNA sequence, what 3 components are required to alter a gene using CRISPR technology? Answer using complete sentences for full credit. (3 points extra) ii) All 3 key components of CRISPR technology described in 1 (i) are macromolecular polymers. Explain what monomeric units compose each of the 3 components. Answer using complete sentences for full credit. (3 points extra)
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Answer #1

The components required to alter a gene using CRISPR technology are macromolecular polymers namely :

1. Cas9,

2. crRNA and

3. trRNA

2. Cas9 is composed of gRNA(guide RNA) and a Cas9 nuclease monomeric unit,

crRNA is composed of spacer/repeat sequences

trRNA consists of small ribosomal subunits

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