The VNTR locus D17S539 has a repeat length of 5 base pairs. Individuals may have from...
The VNTR locus D17S539 has a repeat length of 5 base pairs. Individuals may have from 2 to 50 repeats. The individuals listed below know the sizes of their alleles. Label the gel below to identify which sample was run in each lane.
Jennifer: 128bp, 128bp
Mandy: 48bp, 200bp,
Kathy: 80bp, 300bp
| Name | Name | Name | ||||||
| Answer (bp) | ||||||||
| Answer (bp) | ||||||||
| Answer (bp) | ||||||||
| Answer (bp) | ||||||||
| Answer (bp) | ||||||||
Homework Answers
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The VNTR locus D17S539 has a repeat length of 5 base pairs.
Individuals may have from...
You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in...
You PCR amplify a 500 bp (base pairs) piece of DNA that has
diagnostic value in determining whether a patient has a mutation
within a specific DNA region. You know that this DNA segment of the
“normal” gene does not include an EcoRI restriction site; but the
mutated DNA segment of the same gene contains an EcoRI restriction
site due to a point mutation at the 100th bp from the 5’ DNA end.
After PCR amplification, you subject your DNA...
3) Image from Biotechnology Explorer Kit, BIO RAD Analysis of DNA Fragments The data you entered...
3) Image from Biotechnology Explorer Kit, BIO RAD Analysis of DNA Fragments The data you entered for the lambda Hindill digest were the relative positions of DNA bands of known size. Since the exact size and position of these fragments are known, they can be used as standard reference points to estimate the size of unknown fragment bands. A set of fragments of known sizes is called a molecular weight ruler or standards or marker (or sometimes a ladder because...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
The PCR was a success and your target region of 770 bp in length has been...
The PCR was a success and your target region of 770 bp in length has been amplified. You now plan to digest the DNA amplicon with the restriction enzyme Eael, and clone the resulting longest fragment it into the Eael site of the 5 kb plasmid diagrammed below. 770 bp BamHI 1 200 EcoRI 800 EcoRI 4000 1000 5 kb O /1000 2000 2000 Faal You purify your recombinant plasmid from bacterial cells, and run the plasmid (uncut. or not...
genetic biology 5'-GCATGAGTCTGGTACGCTTTTAAAGC-3' 3-CATGCG-5' IIIII 3. (a) in the sequence above, what enzyme would you need...
genetic biology
5'-GCATGAGTCTGGTACGCTTTTAAAGC-3' 3-CATGCG-5' IIIII 3. (a) in the sequence above, what enzyme would you need to extend the short stretch of nucleotides shown on the bottom strand? (b) Write the sequence of the newly synthesized fragment and label its S' and 3' end. (c) The covalent bond between these adjacent nucleotides is what type of chemical bond? After using a chemical mutagen to generate mutations in a DNA sequence, scientists noted a mutation from C to T at the...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
You PCR amplify a 500 bp (base pairs) piece of DNA that has
diagnostic value in determining whether a patient has a mutation
within a specific DNA region. You know that this DNA segment of the
“normal” gene does not include an EcoRI restriction site; but the
mutated DNA segment of the same gene contains an EcoRI restriction
site due to a point mutation at the 100th bp from the 5’ DNA end.
After PCR amplification, you subject your DNA...
3) Image from Biotechnology Explorer Kit, BIO RAD Analysis of DNA Fragments The data you entered for the lambda Hindill digest were the relative positions of DNA bands of known size. Since the exact size and position of these fragments are known, they can be used as standard reference points to estimate the size of unknown fragment bands. A set of fragments of known sizes is called a molecular weight ruler or standards or marker (or sometimes a ladder because...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
The PCR was a success and your target region of 770 bp in length has been amplified. You now plan to digest the DNA amplicon with the restriction enzyme Eael, and clone the resulting longest fragment it into the Eael site of the 5 kb plasmid diagrammed below. 770 bp BamHI 1 200 EcoRI 800 EcoRI 4000 1000 5 kb O /1000 2000 2000 Faal You purify your recombinant plasmid from bacterial cells, and run the plasmid (uncut. or not...
genetic biology
5'-GCATGAGTCTGGTACGCTTTTAAAGC-3' 3-CATGCG-5' IIIII 3. (a) in the sequence above, what enzyme would you need to extend the short stretch of nucleotides shown on the bottom strand? (b) Write the sequence of the newly synthesized fragment and label its S' and 3' end. (c) The covalent bond between these adjacent nucleotides is what type of chemical bond? After using a chemical mutagen to generate mutations in a DNA sequence, scientists noted a mutation from C to T at the...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
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