Please answer the following question. CIrcle the final answer. Thanks in advance.
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Please answer the following question. CIrcle the final answer.
Thanks in advance.
Suppose a researcher conducts...
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table...
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Identify the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin. Hfr strain Order of gene transfer ikvqw izrbm vkimb 3 Answer Bank Answer...
Three Hfr strains from the same bacterial species are analyzed for their ability to transfer genes...
Three Hfr strains from the same bacterial species are analyzed for their ability to transfer genes to F recipient bacteria. The data shown below list the origin of transfer (oriT) for each strain and give the order of genes, with the first gene on the left and the last gene on the right. Hfr Strain Genes Transferred Hfr 1 Hfr 2 oriT met pro leu gal oriT ala lac thr azi oriT azi trp met pro Hfr 3 Use the...
Sam is working in a genetics lab this summer in order to gain more hands-on experience...
Sam is working in a genetics lab this summer in order to gain more hands-on experience before he applies to graduate school. The professor with whom he is working is particularly interested in studying a new strain of E.coli and assigns Sam an interrupted mating experiment to perform. Sam is attempting to determine the order of 7 different genes on the bacterial chromosome: Ger, Fra, Lod, Moc, Bla, Jat, and Arp. Sam used four different Hfr strains (Hfr 1, Hfr2,...
Please explain step by step... thank you! In order to determine the overall chromosome map for...
Please explain step by step... thank you!
In order to determine the overall chromosome map for E coli, three Hfr strains were obtained from a protrophic but erythromycin resistant F+ ancestor. The start times of transfer during conjugation matings with F- strains carrying auxotrophic, fermentation, and erythromycin- sensitivity markers were determined for each Hfr strain, as listed. A. Using the times for transfer of each marker, draw a unified chromosome map of E coli, showing the order and relative distances...
Genetics Question: I am confused on the second part of the question- determining the information for...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...
This is a Hfr mapping question and I'm completely lost here Basically the bonus is a...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
I am having trouble in understanding this question. The second photo is part b of the question. A...
I am having trouble in understanding this question. The second
photo is part b of the question. Any help would be
appreciated!
Four Hfr strains of E.ole known to transfer thier genetic material during conjugation in different sequences. Given the time of entry of the markers into the F-recipient, construct a genetic map which includes all of these markers, starting with arg as number 1 and phe as number 11.(Not all markers were assayed in each strain, remember the E.coli...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM 5cM 40cM If a diploid female heterozygous for A and D (A D//a d) is testcrossed to a homozygous recessive male (a d/a d), what percent of the progeny are predicted to have the genotype A d/a d? A. 100 B. 75 C. 50 D. 25 E. Can’t be determined 2. Yellow body (y), vermillion eye color (v) and miniature body (m) are recessive...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Identify the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin. Hfr strain Order of gene transfer ikvqw izrbm vkimb 3 Answer Bank Answer...
Three Hfr strains from the same bacterial species are analyzed for their ability to transfer genes to F recipient bacteria. The data shown below list the origin of transfer (oriT) for each strain and give the order of genes, with the first gene on the left and the last gene on the right. Hfr Strain Genes Transferred Hfr 1 Hfr 2 oriT met pro leu gal oriT ala lac thr azi oriT azi trp met pro Hfr 3 Use the...
Sam is working in a genetics lab this summer in order to gain more hands-on experience before he applies to graduate school. The professor with whom he is working is particularly interested in studying a new strain of E.coli and assigns Sam an interrupted mating experiment to perform. Sam is attempting to determine the order of 7 different genes on the bacterial chromosome: Ger, Fra, Lod, Moc, Bla, Jat, and Arp. Sam used four different Hfr strains (Hfr 1, Hfr2,...
Please explain step by step... thank you!
In order to determine the overall chromosome map for E coli, three Hfr strains were obtained from a protrophic but erythromycin resistant F+ ancestor. The start times of transfer during conjugation matings with F- strains carrying auxotrophic, fermentation, and erythromycin- sensitivity markers were determined for each Hfr strain, as listed. A. Using the times for transfer of each marker, draw a unified chromosome map of E coli, showing the order and relative distances...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
I am having trouble in understanding this question. The second
photo is part b of the question. Any help would be
appreciated!
Four Hfr strains of E.ole known to transfer thier genetic material during conjugation in different sequences. Given the time of entry of the markers into the F-recipient, construct a genetic map which includes all of these markers, starting with arg as number 1 and phe as number 11.(Not all markers were assayed in each strain, remember the E.coli...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
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