Question

4. (4 pts) in premature termination of translation. These mutations are not as rare as you might think. For example, there are 8 different sense codons that can be changed to the stop codon UAG by a single point mutation (base change). Nonsense mutations are mutations that introduce a STOP codon in place of a sense codon, and result Using the codon table in the textbook, list these 8 sense codons (and their corresponding amino acid) Consider the following introduction of a nonsense mutation into the provided wild type sequence Transcribe both the wild type and mutant sequence into mRNA and then translate each into its respective polypeptide. I WANT TO SEE POLARITY FOR ALL MOLECULES! Nonsense 5 '-ATGCCGGTCCAGCTT-3 mutation → 3'-TACGGCCAGATCGAA-5 , ← templat - template → 3,-TACGGCCAGGTCGAA-5,

Answer 1

8 sense codons that can be changed to the stop codon UAG by a single point mutation include

UUG - Leucine

UAU – Tyrosine        

UAC – Tyrosine

UCG - Serine

UGG – Tryptophan

AAG – Lysine

CAG - Glutamine

GAG – Glutamic acid

Wild type

Template – 3’ – TACGGCCAGGTCGAA – 5’

mRNA – 5’ – AUGCCGGUCCAGCUU – 3’

Polypeptide sequence – Met-Pro-Val-Gln-Leu

Mutant

Template – 3’ – TACGGCCAGATCGAA-5’

mRNA – 5’ – AUGCCGGUCUAGCUU - 3’

Polypeptide sequence – Met-Pro-Val-Stop