Question

Did I answer the mRNA sequences and Amino acid sequences correctly? What types of mutations are these? How do you do the bottom?  

Part 3. Cystic Fibrosis Directions: Cystic Fibrosis is a disorder where the individuals have long and kidney problems. The disorder is caused by a mutation in one of the individual's genes. Complete the boxes below by finding the mRNA and amino acid sequence Compare the moutant DNA strands to the original strand. Circle the mutation in the tant DNA strands. Classify each mutation 1. Normal Gene: ACCATTA AAGAAAATATCATCTIIGGTGTITCCTATGAT mRNA sequence: UGG WAA MI CULTIA UAG UAG AAA CCA CAA AGG AUIA CUA Amino acid sequence: TRP-STOP-PHE-LEL-LEU-STOP-STOP-LYS-PRO-GIN-ARG-ILE-LEU 2. Mutant Genel: ACCATTA AAGAAAATATCATCGTGT TCCTATGAT mRNA sequence: UGG UAA UUU CUU LUA UAG LAG CCA CAA AGG AUA CUA Amino acid sequence: TRP-STOP-PHE-LEU-LEU-STOP-STOP-PRO-GIN-ARG ILE-LEU Type of Mutation: 3. Mutant Gene 2: ACCATTA AAGAAAAAATCATCTTIGGTGTTTCCTATGAT mRNA sequence UGG UAA UUU CUU UUU UAG LAG AAA CCA CAA AGG AUA CUA Amino acid sequence: TRP-STOP-PHE-LEU-PHE-STOP-STOP-LYS-PRO-GIN-ARG ILE-LEU Type of Mutation: Part 4. Sickle Cell Below are the DNA sequence for the normal hemoglobin protein and the mutant DNA sequence for the sickle cell hemoglobin protein. Normal Hemoglobin: GGGCTICTTIIT Sickle Cell Hemoglobin: GGGCATCTIIII A. Transcribe and translate the normal and sickle cell DNA. NORMAL mRNA sequence: NORMAL Amino acid sequence: MUTANT MRNA sequence: MUTANT Amino acid sequence: B. Identify this type of mutation and explain why it is. C. If the mutant DNA sequence was GGGCTICTIAAA, would this mutant DNA result in sickle cell hemoglobin? Explain your answer.

Answer 1

PART 3

2) DELETION OF TWO THYMINE(DELETION MUTATION)

3)ADDIDITION OF TWO ADENINE(ADDITION MUTATION)

PART 4

A) NORMAL mRNA SEQUENCE-CCC GAA GAA AAA

NORMAL AMINO ACID SEQUENCE-pro-glu-glu-lys

MUTATED mRNA SEQUENCE-CCC GUA GAA AAA

MUTATED AMINO ACID SEQUENCE-pro-val-glu-lys

B) SUBSTITUTION MUTATION

C) If the mutation DNA sequence was GGG CTT CTT AAA THEN IT RESULT IN SICKLE CELL ANEMIA BECAUSE MUTATION CHANGES THE LYSINE TO PHENYL ALANINE AND LYSINE IS A CHARGED AMINO ACID WHERE AS PHENYALANINE IS A NON POLAR AMINO ACID, WHICH TENDS TO REMAIN IN THE HYDROPHOBIC REGION.