When lactose is absent .
1- repressor will bind to operator.
2- gene will be swtiched off .
When lactose is present for transcription.
1- repressor will bind to lactose and freeing the operator site .
2- gene will be swtiched on for transcription .
When lactose is absent: 1. Is the repressor protein bound to the operator? 2. Is gene...
if expression of a gene is regulated by stopping a repressor protein from binding to an operator, transcription could also be described as affected by 01. Negative control 2. Induction 3. Positive control 1. Repression
10. Using the lactose operon as an example, diagram and explain gene expression through the action of a promoter, an operator, structural genes, and a repressor protein when lactose is present and when lactose is absent. 11. Define and give examples of epigenetic control of gene expression.
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
The tryptophan operator... A is an allosteric protein. binds to the tryptophan repressor when the repressor is bound to tryptophan 0 is required for production of the mRNA encoded by the tryptophan operon 1. is important for the production of the tryptophan repressor.
The diagram below illustrates the LAC operon in its OFF state when the inducer molecule —lactose—is absent. Predict the ways in which the following conditions will affect the transcription of the lactose-utilization genes. OPERON Regulatory Promoter Operator_ gene Lactose-utilization genes DNA mRNA RNA polymerase cannot attach to promoter Active repressor Protein If a mutation in the regulatory gene results in a misfolding of the repressor protein so that it can no longer bind DNA, the lactose-utilization genes O Will be...
Consider an E. coli strain carrying a mutation in crp (encoding the catabolite activator protein) that prevents CAP from binding CAMP. Predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels...
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site in DNA, thus allowing for expression of the genes coded by the Lac Operon. However, lactose cannot cross the bacterial cell membrane without the help of the Permease protein channel, which is coded by the lacY gene in the Lac Operon. So, it appears that we have a case of "what's first, the chicken or the egg?" - how can lactose enter the cell...
Consider what you know about the lac operon, and predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels of transcription. and the activator is b. When lactose and glucose are...
here is the diagram ecause there is no repressor protein attached to the operator, what enzyme con attach to the promoter and move past the operator to transcribe the structural genes? Color this enzyme pink "color the repressor gene purple and the repressor protein it codes for red. Examine the shape of the repressor protein. 45) Is it an active or inactiverepressor protein? The diagram below shows the trp operon when turned off." Repressor gene Promoter Operator Structural genes DNA...
Question 51 (1 point) The lactose repressor protein a) is activated by binding lactose b) is inactivated by binding lactose C) is transcribed with the structural lac genes d) requires lactose for its transcription e) None of the choices are correct.