Question

Hexokinase: as discussed in class, hexokinase phosphorylates glucose by hydrolyzing ATP. Specifically, it transfers a phosphate from ATP to the C6 atom in glucose. Write the mechanism that is facilitated by hexokinase, include the TS. What forces or strategies would stabilize the TS and which amino acids would likely participate? Be specific about which amino acids would be useful in which particular role.

Answer 1

Hexokinase catalyze the reaction of phosphryl group transfer, it undergose the conformational change after binding to glucose. When hexokinase binds to glucose molecule, the gamma- phosphoryl group of ATP molecule is is tratransfrred to the oxygen at the C-6 of glucos, for this magnesium ion requires as the reactive form of ATP. This reaction require direct nucleophillic attack of hydroxyl group, and result in the production of glucose 6 phosphate and ADP molecule, the active side amino acid residue for hexokinase are aspartate, lysine, arsenic, threonine and glutamate.