Homework Answers
1. The enzyme hexokinase transfers a phosphate from ATP to glucose,to make ADP and glucose-6-phosphate . Which of the following is a substrate?
Ans. Glucose.
2. The enzyme hexokinase transfers a phosphate from ATP to glucose, to make ADP and glucose-6-phosphate. Which of the following is a product?
Ans. Glucose-6-phosphate
Explanation:
Substrate is a molecule on which an enzyme acts. Substrate binds with the active site of the enzyme to form enzyme-substrate complex.
Enzyme Converts the substrate into product.
Enzyme helps to convert complex molecules into simpler molecules so that it can be absorbed by the body. During glycolysis(breakdown of glucose) hexokinase enzyme phosphorylates glucose(substrate)forming glucose-6-phosphate(product).
Hexokinase enzyme is responsible for glucose metabolism and maintaining intracellular glucose concentration.
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Question 1 1 pts The enzyme hexokinase transfers a phosphate from ATP to glucose, to make...
The enzyme hexokinase catalyzes the following reaction: Glucose + ATP ↔ Glucose 6-phosphate + ADP For...
The enzyme hexokinase catalyzes the following reaction: Glucose + ATP ↔ Glucose 6-phosphate + ADP For this reaction, ΔG° = +16.7 kJ/mol a. Calculate the equilibrium constant for this reaction at 25 °C. Is this reaction spontaneous under standard conditions at this temperature? Calculate the ΔG if the reaction temperature was 25 °C and the concentrations are: glucose, 55.0 mM,; ATP, 5.00 mM; ADP, 1.00 mM; and glucose 6-Phosphate, 0.100 mM. Would the reaction be spontaneous under the conditions of...
Hexokinase: as discussed in class, hexokinase phosphorylates glucose by hydrolyzing ATP. Specifically, it transfers a phosphate...
Hexokinase: as discussed in class, hexokinase phosphorylates glucose by hydrolyzing ATP. Specifically, it transfers a phosphate from ATP to the C6 atom in glucose. Write the mechanism that is facilitated by hexokinase, include the TS. What forces or strategies would stabilize the TS and which amino acids would likely participate? Be specific about which amino acids would be useful in which particular role.
the enzyme hexokinase can catalyze the following reaction: fructose + ATP -> fructose 6 phosphate +...
the enzyme hexokinase can catalyze the following
reaction:
fructose + ATP -> fructose 6 phosphate + adp
if the deltaG of hydrolysis of fructose 6 phosphate to
fructose + Pi is -13.8 kj/mol and hydrolysis of atp to adp + pi is
-30kj/mol, calculate Keq for the reaction above (R = 8.31 j mol-1
K-1, T = 298 k
3a. The enzyme hexokinase can catalyze the following reaction: Fructose + ATP → Fructose-6-phosphate + ADP If the ΔGo of hydrolysis...
7.5 Hexokinase catalyzes the reaction: ATP + Glucose A glucose-6-phosphate + ADP AG - -4 562...
7.5 Hexokinase catalyzes the reaction: ATP + Glucose A glucose-6-phosphate + ADP AG - -4 562 cal / mol Keg = 2.21 x 103 Calculate the concentration of glucose-6-phosphate necessary to force the hexokinase reaction to go in the opposite direction (towards the synthesis of glucose and ATP) in the presence of 10-5 M glucose, 10-3 M ATP and 10-4 M ADP.
Hexokinase catalyzes the following reaction in glycolysis: Glucose + ATP ← → glucose-6-phosphate + ADP The...
Hexokinase catalyzes the following reaction in glycolysis: Glucose + ATP ← → glucose-6-phosphate + ADP The standard free-energy change for this reaction in the direction written is -16.7 kJ/mol. The concentrations of the related reaction components in the hepatocyte of a mammalian cell are: Glucose: 6 mM Glucose-6-phosphate: 0.074 mM ATP: 1.00 mM ADP: 0.25 mM What is the actual free-energy change for the reaction at body temperature (37 C)?
Hexokinase catalyzes the first step of glycolysis, in which glucose is phosphorylated to form glucose-6-phosphate. Which...
Hexokinase catalyzes the first step of glycolysis, in which glucose is phosphorylated to form glucose-6-phosphate. Which of these statements are accurate? The conformational shift that occurs when glucose, but nox water, enters the active site prevents water from hydrolyzing ATP Most kinases require the presence of a monovalent metal ion cofactor to prevent ATP hydrolysis. Hexokinase transfers the terminal phosphate of ATP to carbon 3 of glucose. Hexokinase consists of two domains, or lobes, that come together when glucose and...
1. Hexokinase catalyzes the ATP-dependent phosphorylation of a broad spectrum of 6- carbon sugars. In the...
1. Hexokinase catalyzes the ATP-dependent phosphorylation of a broad spectrum of 6- carbon sugars. In the reaction involving glucose and ATP, glucose-6-phosphate (G6P) and ADP are formed. D-Glucose + ATP G6P + ADP + Ht (AG",--20.9 kJ/mol). The enzyme specifically recognizes the acetal form of glucose, (a-D-glucopyranose. a. Draw the linear form of glucose using a Fischer projection and draw the structure of (a-D-glucopyranose. (5 pts each) b. Provide the net reaction for glycolysis. (5 pts) c. Assuming lysine acts...
the following questions Fructose-1.6- Fructose-6 ADP phosphate phosphate ATP ADP diphosphate Glucose-6 F ATP Glucose-→ Glucose-p)→...
the following questions Fructose-1.6- Fructose-6 ADP phosphate phosphate ATP ADP diphosphate Glucose-6 F ATP Glucose-→ Glucose-p)→ Fructose-P P-Fructose-(P FIGURE 5.7 glyceraldyde 3-phosphate P) ADP H-C-OH H20-P 1,3 diphospho- glycerate CH20-P 3 phospho glycerate phosphoenol pyruvate 15) Which step in Figure 5.7 shows a split of one molecule into two smaller molecules? 15) 4) 2 B) 4 )6 Dy H E) 5 16) Which step in Figure 5.7 shows a reaction involving a dehydrogenase? D) 8 A) 2 C) 6 E)5...
gets reduced 20. glucose + ATP > glucose-6 phosphate +ADP In the above reaction, - A....
gets reduced 20. glucose + ATP > glucose-6 phosphate +ADP In the above reaction, - A. ADPIATP B. ATP/glucose C. glucose/glucose-6 phosphate D. glucose6-phosphate/glucose E. glucose/ATP 21. Which of the following is a net product of glycolysis? A. ATP B. NAD+ C. pyruvate D. CO2 E. A&C 22. Substrate-level phosphorylation occurs in the process of A. Oxidative phosphorylation B. electron transport chain C. glycolysis D. ATP-synthase function E. C&D 23. What should your hypothesis be for "Do yeast produce ethanol...
2. As an alternative, people developed another enzyme mediated assay to measure glucose concentration as below:...
2. As an alternative, people developed another enzyme mediated assay to measure glucose concentration as below: Hexokinase Glucose + ATP Glucose-6-Phosphate + ADP G6PDH G6P + NAD 6-Phosphogluconate + NADH Based on the information above and what you learned so far, how exactly can you determine the glucose concentration from this method? Hint: spectrophotometrically (10 pts)
Hexokinase: as discussed in class, hexokinase phosphorylates glucose by hydrolyzing ATP. Specifically, it transfers a phosphate from ATP to the C6 atom in glucose. Write the mechanism that is facilitated by hexokinase, include the TS. What forces or strategies would stabilize the TS and which amino acids would likely participate? Be specific about which amino acids would be useful in which particular role.
the enzyme hexokinase can catalyze the following
reaction:
fructose + ATP -> fructose 6 phosphate + adp
if the deltaG of hydrolysis of fructose 6 phosphate to
fructose + Pi is -13.8 kj/mol and hydrolysis of atp to adp + pi is
-30kj/mol, calculate Keq for the reaction above (R = 8.31 j mol-1
K-1, T = 298 k
3a. The enzyme hexokinase can catalyze the following reaction: Fructose + ATP → Fructose-6-phosphate + ADP If the ΔGo of hydrolysis...
Hexokinase catalyzes the first step of glycolysis, in which glucose is phosphorylated to form glucose-6-phosphate. Which of these statements are accurate? The conformational shift that occurs when glucose, but nox water, enters the active site prevents water from hydrolyzing ATP Most kinases require the presence of a monovalent metal ion cofactor to prevent ATP hydrolysis. Hexokinase transfers the terminal phosphate of ATP to carbon 3 of glucose. Hexokinase consists of two domains, or lobes, that come together when glucose and...
1. Hexokinase catalyzes the ATP-dependent phosphorylation of a broad spectrum of 6- carbon sugars. In the reaction involving glucose and ATP, glucose-6-phosphate (G6P) and ADP are formed. D-Glucose + ATP G6P + ADP + Ht (AG",--20.9 kJ/mol). The enzyme specifically recognizes the acetal form of glucose, (a-D-glucopyranose. a. Draw the linear form of glucose using a Fischer projection and draw the structure of (a-D-glucopyranose. (5 pts each) b. Provide the net reaction for glycolysis. (5 pts) c. Assuming lysine acts...
the following questions Fructose-1.6- Fructose-6 ADP phosphate phosphate ATP ADP diphosphate Glucose-6 F ATP Glucose-→ Glucose-p)→ Fructose-P P-Fructose-(P FIGURE 5.7 glyceraldyde 3-phosphate P) ADP H-C-OH H20-P 1,3 diphospho- glycerate CH20-P 3 phospho glycerate phosphoenol pyruvate 15) Which step in Figure 5.7 shows a split of one molecule into two smaller molecules? 15) 4) 2 B) 4 )6 Dy H E) 5 16) Which step in Figure 5.7 shows a reaction involving a dehydrogenase? D) 8 A) 2 C) 6 E)5...
gets reduced 20. glucose + ATP > glucose-6 phosphate +ADP In the above reaction, - A. ADPIATP B. ATP/glucose C. glucose/glucose-6 phosphate D. glucose6-phosphate/glucose E. glucose/ATP 21. Which of the following is a net product of glycolysis? A. ATP B. NAD+ C. pyruvate D. CO2 E. A&C 22. Substrate-level phosphorylation occurs in the process of A. Oxidative phosphorylation B. electron transport chain C. glycolysis D. ATP-synthase function E. C&D 23. What should your hypothesis be for "Do yeast produce ethanol...
2. As an alternative, people developed another enzyme mediated assay to measure glucose concentration as below: Hexokinase Glucose + ATP Glucose-6-Phosphate + ADP G6PDH G6P + NAD 6-Phosphogluconate + NADH Based on the information above and what you learned so far, how exactly can you determine the glucose concentration from this method? Hint: spectrophotometrically (10 pts)
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